ARG65369

anti-CD3 epsilon antibody [145-2C11]

anti-CD3 epsilon antibody [145-2C11] for CyTOF®-candidate,Flow cytometry,Functional study,ICC/IF,IHC-Frozen sections,Immunoprecipitation and Mouse

Cancer antibody; Developmental Biology antibody; Immune System antibody; Lymphocyte Marker antibody; Inflammatory Cell Marker antibody; T-cell Marker antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody

Overview

Product Description Hamster Monoclonal antibody [145-2C11] recognizes CD3 epsilon
Tested Reactivity Ms
Tested Application CyTOF®-candidate, FACS, FuncSt, ICC/IF, IHC-Fr, IP
Specificity The clone 145-2C11 reacts with mouse CD3 (epsilon subunit). This antibody is commonly used as a phenotypic marker for mouse T cells.
Host Hamster
Clonality Monoclonal
Clone 145-2C11
Isotype IgG
Target Name CD3 epsilon
Antigen Species Mouse
Immunogen Mouse BM10-37 cytotoxic T lymphocytes.
Conjugation Un-conjugated
Alternate Names T-cell surface antigen T3/Leu-4 epsilon chain; T3E; TCRE; T-cell surface glycoprotein CD3 epsilon chain; IMD18; CD antigen CD3e

Application Instructions

Application Suggestion
Tested Application Dilution
CyTOF®-candidateAssay-dependent
FACS1 - 2 µg/10^6 cells
FuncStAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IP1 - 2 µg / 100 - 500 µg of protein in 1 ml lysate
Application Note Functional studies: Induction of T cell activation, proliferation or apoptosis (depending on conditions); in vivo T cell depletion.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified by protein A
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12501 Mouse CD3E

Swiss-port # P22646 Mouse T-cell surface glycoprotein CD3 epsilon chain

Gene Symbol Cd3e
Gene Full Name CD3 antigen, epsilon polypeptide
Background CD3 subunit complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins superfamily encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
Function CD3: Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128). [UniProt]
Highlight Related products:
CD3 antibodies; CD3 ELISA Kits; CD3 Duos / Panels; CD3 recombinant proteins; Anti-Hamster IgG secondary antibodies;
Related news:
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Tumor-Infiltrating Lymphocytes (TILs)
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Research Area Cancer antibody; Developmental Biology antibody; Immune System antibody; Lymphocyte Marker antibody; Inflammatory Cell Marker antibody; T-cell Marker antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody
Calculated MW 23 kDa

Clone References

Statins induce regulatory T cell recruitment via a CCL1 dependent pathway.

IHC-Fr / Mouse

Mira E et al.
J Immunol.,  (2008)

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HSP70 vaccine in combination with gene therapy with plasmid DNA encoding sPD-1 overcomes immune resistance and suppresses the progression of pulmonary metastatic melanoma.

Geng H et al.
Int J Cancer.,  (2006)

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Functional analysis of B and T lymphocyte attenuator engagement on CD4+ and CD8+ T cells.

Krieg C et al.
J Immunol.,  (2005)

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hr_line

CCR5-deficient mice develop experimental autoimmune uveoretinitis in the context of a deviant effector response.

Takeuchi A et al.
Invest Ophthalmol Vis Sci.,  (2005)

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Regulation of NK cell function in vivo by the dose of tumour transplanted in the peritoneum.

FACS / Rat

Das S et al.
Immunol Lett.,  (2002)

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