ARG65722

anti-CD3 zeta phospho (Tyr142) antibody [EM-54]

anti-CD3 zeta phospho (Tyr142) antibody [EM-54] for Flow cytometry,Western blot and Human,Mouse

Developmental Biology antibody; Immune System antibody

Overview

Product Description Mouse Monoclonal antibody [EM-54] recognizes CD3 zeta phospho (Tyr142)
Tested Reactivity Hu, Ms
Tested Application FACS, WB
Specificity This antibody recognizes phosphorylated tyrosine 142 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.
Host Mouse
Clonality Monoclonal
Clone EM-54
Isotype IgG1
Target Name CD3 zeta
Antigen Species Human
Immunogen Phosphospecific peptide around Tyr142 of Human CD3 zeta.
Conjugation Un-conjugated
Alternate Names IMD25; CD3Z; CD3-ZETA; T-cell surface glycoprotein CD3 zeta chain; CD3Q; T-cell receptor T3 zeta chain; CD3H; TCRZ; T3Z; CD antigen CD247

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 4 µg/ml
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12503 Mouse CD247

GeneID: 919 Human CD247

Swiss-port # P20963 Human T-cell surface glycoprotein CD3 zeta chain

Swiss-port # P24161 Mouse T-cell surface glycoprotein CD3 zeta chain

Gene Symbol CD247
Gene Full Name CD247 molecule
Background The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering. [UniProt]
Highlight Related products:
CD3 antibodies; CD3 ELISA Kits; CD3 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
New antibody panels and duos for Tumor immune microenvironment
Tumor-Infiltrating Lymphocytes (TILs)
Research Area Developmental Biology antibody; Immune System antibody
Calculated MW 19 kDa
PTM Phosphorylated on Tyr residues after T-cell receptor triggering.