ARG65722
anti-CD3 zeta phospho (Tyr142) antibody [EM-54]
anti-CD3 zeta phospho (Tyr142) antibody [EM-54] for Flow cytometry,Western blot and Human,Mouse
Developmental Biology antibody; Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [EM-54] recognizes CD3 zeta phospho (Tyr142) |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FACS, WB |
| Specificity | This antibody recognizes phosphorylated tyrosine 142 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | EM-54 |
| Isotype | IgG1 |
| Target Name | CD3 zeta |
| Antigen Species | Human |
| Immunogen | Phosphospecific peptide around Tyr142 of Human CD3 zeta. |
| Conjugation | Un-conjugated |
| Alternate Names | IMD25; CD3Z; CD3-ZETA; T-cell surface glycoprotein CD3 zeta chain; CD3Q; T-cell receptor T3 zeta chain; CD3H; TCRZ; T3Z; CD antigen CD247 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P20963 Human T-cell surface glycoprotein CD3 zeta chain Swiss-port # P24161 Mouse T-cell surface glycoprotein CD3 zeta chain |
|---|---|
| Gene Symbol | CD247 |
| Gene Full Name | CD247 molecule |
| Background | The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering. [UniProt] |
| Highlight | Related products: CD3 antibodies; CD3 ELISA Kits; CD3 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) |
| Research Area | Developmental Biology antibody; Immune System antibody |
| Calculated MW | 19 kDa |
| PTM | Phosphorylated on Tyr residues after T-cell receptor triggering. |
