ARG65443

anti-CD3 zeta phospho (Tyr72) antibody [EM-26]

anti-CD3 zeta phospho (Tyr72) antibody [EM-26] for Flow cytometry,ICC/IF,Western blot and Human,Mouse

Developmental Biology antibody; Immune System antibody

Overview

Product Description Mouse Monoclonal antibody [EM-26] recognizes CD3 zeta phospho (Tyr72)
Tested Reactivity Hu, Ms
Tested Application FACS, ICC/IF, WB
Specificity The clone EM-26 recognizes phosphorylated tyrosine 72 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.
Host Mouse
Clonality Monoclonal
Clone EM-26
Isotype IgG1
Target Name CD3 zeta
Antigen Species Mouse
Immunogen A phospho specific peptide corresponding to the amino acids surrounding tyrosine 72 of mouse CD3 zeta linked to KLH
Conjugation Un-conjugated
Alternate Names IMD25; CD3Z; CD3-ZETA; T-cell surface glycoprotein CD3 zeta chain; CD3Q; T-cell receptor T3 zeta chain; CD3H; TCRZ; T3Z; CD antigen CD247

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 9 µg/ml
ICC/IFAssay-dependent
WB2 - 5 µg/ml
Application Note WB: Non-reducing condition recommended.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control FACS: Jurkat + pervanadate; T-cells from lymph nodes of OT-1 mouse + pervanadate.
WB: Jurkat + pervanadate; Splenocyte of Balb/c or F1 mouse + pervanadate.

Properties

Form Liquid
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12503 Mouse CD247

GeneID: 919 Human CD247

Swiss-port # P20963 Human T-cell surface glycoprotein CD3 zeta chain

Swiss-port # P24161 Mouse T-cell surface glycoprotein CD3 zeta chain

Gene Symbol Cd247
Gene Full Name CD247 antigen
Background CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
Function Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering. [UniProt]
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Research Area Developmental Biology antibody; Immune System antibody
Calculated MW 19 kDa
PTM Phosphorylated on Tyr residues after T-cell receptor triggering.