ARG53824
anti-CD30 antibody [MEM-268] (APC)
anti-CD30 antibody [MEM-268] (APC) for Flow cytometry and Human
Cancer antibody; Immune System antibody
Overview
Product Description | APC-conjugated Mouse Monoclonal antibody [MEM-268] recognizes CD30 |
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Tested Reactivity | Hu |
Tested Application | FACS |
Specificity | The clone MEM-268 recognizes extracellular part of CD30 (Ki-1 antigen), a 105 kDa single chain glycoprotein expressed on Hodgkin's and Reed-Sternberg cells; it is also found in Burkitt's lymphomas, virus-infected T and B lymphocytes, and on normal B and T lymphocytes after activation (T lymphocytes that produce Th2-type cytokines and on CD4+/CD8+ T lymphocytes that co-express CD45RO and the IL4 receptor). |
Host | Mouse |
Clonality | Monoclonal |
Clone | MEM-268 |
Isotype | IgG |
Target Name | CD30 |
Immunogen | Expression vector containing CD30 cDNA (booster suspension of THP-1 cell line) |
Conjugation | APC |
Alternate Names | Tumor necrosis factor receptor superfamily member 8; Ki-1 antigen; CD30; Ki-1; Lymphocyte activation antigen CD30; CD antigen CD30; D1S166E; CD30L receptor |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
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Purification Note | The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
Buffer | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
Preservative | 15 mM Sodium azide |
Stabilizer | 0.2% (w/v) high-grade protease free BSA |
Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links |
Swiss-port # P28908 Human Tumor necrosis factor receptor superfamily member 8 |
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Gene Symbol | TNFRSF8 |
Gene Full Name | tumor necrosis factor receptor superfamily, member 8 |
Background | CD30 is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
Function | CD30 is a receptor for TNFSF8/CD30L (PubMed:8391931). May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B (PubMed:8999898). [UniProt] |
Research Area | Cancer antibody; Immune System antibody |
Calculated MW | 64 kDa |
PTM | Phosphorylated on serine and tyrosine residues. |