ARG62809
anti-CD30 antibody [MEM-268]
anti-CD30 antibody [MEM-268] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections and Human
Cancer antibody; Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [MEM-268] recognizes CD30 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-P |
| Specificity | The clone MEM-268 recognizes extracellular part of CD30 (Ki-1 antigen), a 105 kDa single chain glycoprotein expressed on Hodgkin's and Reed-Sternberg cells; it is also found in Burkitt's lymphomas, virus-infected T and B lymphocytes, and on normal B and T lymphocytes after activation (T lymphocytes that produce Th2-type cytokines and on CD4+/CD8+ T lymphocytes that co-express CD45RO and the IL4 receptor). |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-268 |
| Isotype | IgG |
| Target Name | CD30 |
| Immunogen | Expression vector containing CD30 cDNA (booster suspension of THP-1 cell line) |
| Conjugation | Un-conjugated |
| Alternate Names | Tumor necrosis factor receptor superfamily member 8; Ki-1 antigen; CD30; Ki-1; Lymphocyte activation antigen CD30; CD antigen CD30; D1S166E; CD30L receptor |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | IHC-P: Thymus |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P28908 Human Tumor necrosis factor receptor superfamily member 8 |
|---|---|
| Gene Symbol | TNFRSF8 |
| Gene Full Name | tumor necrosis factor receptor superfamily, member 8 |
| Background | CD30 is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| Function | CD30 is a receptor for TNFSF8/CD30L (PubMed:8391931). May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B (PubMed:8999898). [UniProt] |
| Highlight | Related products: CD30 antibodies; CD30 ELISA Kits; Anti-Mouse IgG secondary antibodies; Related news: Lymphoma |
| Research Area | Cancer antibody; Immune System antibody |
| Calculated MW | 64 kDa |
| PTM | Phosphorylated on serine and tyrosine residues. |
Clone References
Development and validation of a fluorescent microsphere immunoassay for soluble CD30 testing.
