ARG62809

anti-CD30 antibody [MEM-268]

anti-CD30 antibody [MEM-268] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections and Human

Cancer antibody; Immune System antibody

Overview

Product Description Mouse Monoclonal antibody [MEM-268] recognizes CD30
Tested Reactivity Hu
Tested Application FACS, IHC-P
Specificity The clone MEM-268 recognizes extracellular part of CD30 (Ki-1 antigen), a 105 kDa single chain glycoprotein expressed on Hodgkin's and Reed-Sternberg cells; it is also found in Burkitt's lymphomas, virus-infected T and B lymphocytes, and on normal B and T lymphocytes after activation (T lymphocytes that produce Th2-type cytokines and on CD4+/CD8+ T lymphocytes that co-express CD45RO and the IL4 receptor).
Host Mouse
Clonality Monoclonal
Clone MEM-268
Isotype IgG
Target Name CD30
Immunogen Expression vector containing CD30 cDNA (booster suspension of THP-1 cell line)
Conjugation Un-conjugated
Alternate Names Tumor necrosis factor receptor superfamily member 8; Ki-1 antigen; CD30; Ki-1; Lymphocyte activation antigen CD30; CD antigen CD30; D1S166E; CD30L receptor

Application Instructions

Application Suggestion
Tested Application Dilution
FACS5 µg/ml
IHC-P5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control IHC-P: Thymus

Properties

Form Liquid
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 943 Human TNFRSF8

Swiss-port # P28908 Human Tumor necrosis factor receptor superfamily member 8

Gene Symbol TNFRSF8
Gene Full Name tumor necrosis factor receptor superfamily, member 8
Background CD30 is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Function CD30 is a receptor for TNFSF8/CD30L (PubMed:8391931). May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B (PubMed:8999898). [UniProt]
Highlight Related products:
CD30 antibodies; CD30 ELISA Kits; Anti-Mouse IgG secondary antibodies;
Related news:
Lymphoma
Research Area Cancer antibody; Immune System antibody
Calculated MW 64 kDa
PTM Phosphorylated on serine and tyrosine residues.

Clone References

Development and validation of a fluorescent microsphere immunoassay for soluble CD30 testing.

Pavlov I et al.
Clin Vaccine Immunol.,  (2009)

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