ARG62810

anti-CD30 antibody [MEM-268] (FITC)

anti-CD30 antibody [MEM-268] (FITC) for Flow cytometry and Human

Cancer antibody; Immune System antibody

Overview

Product Description FITC-conjugated Mouse Monoclonal antibody [MEM-268] recognizes CD30
Tested Reactivity Hu
Tested Application FACS
Specificity The clone MEM-268 recognizes extracellular part of CD30 (Ki-1 antigen), a 105 kDa single chain glycoprotein expressed on Hodgkin's and Reed-Sternberg cells; it is also found in Burkitt's lymphomas, virus-infected T and B lymphocytes, and on normal B and T lymphocytes after activation (T lymphocytes that produce Th2-type cytokines and on CD4+/CD8+ T lymphocytes that co-express CD45RO and the IL4 receptor).
Host Mouse
Clonality Monoclonal
Clone MEM-268
Isotype IgG
Target Name CD30
Immunogen Expression vector containing CD30 cDNA (booster suspension of THP-1 cell line)
Conjugation FITC
Alternate Names Tumor necrosis factor receptor superfamily member 8; Ki-1 antigen; CD30; Ki-1; Lymphocyte activation antigen CD30; CD antigen CD30; D1S166E; CD30L receptor

Application Instructions

Application Suggestion
Tested Application Dilution
FACS20 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 943 Human TNFRSF8

Swiss-port # P28908 Human Tumor necrosis factor receptor superfamily member 8

Gene Symbol TNFRSF8
Gene Full Name tumor necrosis factor receptor superfamily, member 8
Background CD30 is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Function CD30 is a receptor for TNFSF8/CD30L (PubMed:8391931). May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B (PubMed:8999898). [UniProt]
Research Area Cancer antibody; Immune System antibody
Calculated MW 64 kDa
PTM Phosphorylated on serine and tyrosine residues.

Clone References

Development and validation of a fluorescent microsphere immunoassay for soluble CD30 testing.

Pavlov I et al.
Clin Vaccine Immunol.,  (2009)

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