ARG42422

anti-CD300a + CD300c antibody [MEM-260] (APC)

anti-CD300a + CD300c antibody [MEM-260] (APC) for Flow cytometry and Human

Overview

Product Description APC-conjugated Mouse Monoclonal antibody [MEM-260] recognizes CD300a + CD300c
Tested Reactivity Hu
Tested Application FACS
Specificity The antibody MEM-260 reacts with an extracellular epitope of CD300a, a 60 kDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25% of circulating T cells and 15% of circulating B cells.
Host Mouse
Clonality Monoclonal
Clone MEM-260
Isotype IgG1
Target Name CD300a + CD300c
Antigen Species Human
Immunogen HPB Human acute lymphoid leukemia cell line.
Conjugation APC
Alternate Names CMRF-35-H9; Inhibitory receptor protein 60; NK inhibitory receptor; CMRF35-H; CMRF35H9; CD antigen CD300a; IRC1/IRC2; IGSF12; CMRF35H; IRp60; IgSF12; CMRF35-like molecule 8; CMRF35-H9; CMRF-35H; CD300 antigen-like family member A; CLM-8; IRC1; IRC2; Immunoglobulin superfamily member 12

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 100 µl of whole blood or 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified
Buffer PBS and 15 mM Sodium azide.
Preservative 15 mM Sodium azide
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11314 Human CD300A

Swiss-port # Q9UGN4 Human CMRF35-like molecule 8

Gene Symbol CD300A
Gene Full Name CD300a molecule
Background This gene encodes a member of the CD300 glycoprotein family of cell surface proteins found on leukocytes involved in immune response signaling pathways. This gene is located on chromosome 17 in a cluster with all but one of the other family members. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]
Function Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation (PubMed:10746781, PubMed:16339535, PubMed:9701027). Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 but not TRIF through activation of PTPN6 (PubMed:22043923). [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. [UniProt]
Calculated MW 33 kDa
PTM Phosphorylated on tyrosine.

N-glycosylated. [UniProt]