ARG53826
anti-CD300a + CD300c antibody [MEM-260] (PE)
anti-CD300a + CD300c antibody [MEM-260] (PE) for Flow cytometry and Human
Immune System antibody
Overview
| Product Description | PE-conjugated Mouse Monoclonal antibody [MEM-260] recognizes CD300a + CD300c |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS |
| Specificity | The clone MEM-260 reacts with CD300a, a 60 kDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25% of circulating T cells and 15% of circulating B cells. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-260 |
| Isotype | IgG1 |
| Target Name | CD300a + CD300c |
| Antigen Species | Human |
| Immunogen | HPB human acute lymphoid leukemia cell line |
| Conjugation | PE |
| Alternate Names | CMRF-35-H9; Inhibitory receptor protein 60; NK inhibitory receptor; CMRF35-H; CMRF35H9; CD antigen CD300a; IRC1/IRC2; IGSF12; CMRF35H; IRp60; IgSF12; CMRF35-like molecule 8; CMRF35-H9; CMRF-35H; CD300 antigen-like family member A; CLM-8; IRC1; IRC2; Immunoglobulin superfamily member 12 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification Note | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| Buffer | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| Preservative | 15 mM Sodium azide |
| Stabilizer | 0.2% (w/v) high-grade protease free BSA |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD300A |
| Gene Full Name | CD300a molecule |
| Background | CD300a (CMRF-35H, IRp60) is a non-MHC-specific inhibitory receptor of immunoglobulin superfamily, which contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that associate with SH2-containing phosphatases SHP-1 and SHP-2. CD300a is expressed on many cell types including T cells, NK cells, neutrophils, eosinophils or mast cells. Its triggering inhibits activating signals such as those of IL5, GM-CSF or eotaxin, as well as supresses mast cell degranulation or NK cell cytotoxic activity. |
| Function | Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation. [UniProt] |
| Research Area | Immune System antibody |
| Calculated MW | 33 kDa |
| PTM | Phosphorylated on tyrosine. N-glycosylated. |
