ARG65552

anti-CD300e antibody [UP-H2]

anti-CD300e antibody [UP-H2] for Flow cytometry,Immunoprecipitation,Western blot,Functional study and Human

Immune System antibody

Overview

Product Description Mouse Monoclonal antibody [UP-H2] recognizes CD300e
Tested Reactivity Hu
Tested Application FACS, FuncSt, IP, WB
Specificity The clone UP-H2 recognizes CD300e / IREM-2, a 32 kDa glycoprotein expressed by mature monocytes and peripheral blood myeloid dendritic cells.
Host Mouse
Clonality Monoclonal
Clone UP-H2
Isotype IgG1
Target Name CD300e
Immunogen CD300e-HA-transfected cells
Conjugation Un-conjugated
Alternate Names Poly-Ig receptor 2; Immune receptor expressed on myeloid cells 2; IREM-2; CD300LE; CMRF35-A5; CD antigen CD300e; CLM2; IREM2; CMRF35-like molecule 2; PIgR2; CLM-2; Polymeric immunoglobulin receptor 2; PIgR-2; CD300 antigen-like family member E

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 4 µg/ml
FuncStAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note Functional studies: Stimulation.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 342510 Human CD300E

Swiss-port # Q496F6 Human CMRF35-like molecule 2

Gene Symbol CD300E
Gene Full Name CD300e molecule
Background CD300e / IREM-2 (immune receptor expressed by myeloid cells 2), also known as CLM2 or LMIR6, is a monomeric transmembrane glycoprotein with a single extracellular immunoglobulin-like domain. Intracellularly it associates with DAP-12, an ITAM-containing adaptor molecule. CD300e is expressed on mature monocytes and peripheral blood myeloid dendritic cells. Its crosslinking leads to release of pro-inflammatory cytokines, and increased expression of activation markers.
Function Probably acts as an activating receptor. [UniProt]
Research Area Immune System antibody
Calculated MW 23 kDa
PTM N-glycosylated.

Clone References

Molecular characterization of a novel immune receptor restricted to the monocytic lineage.

Aguilar H et al.
J Immunol.,  (2004)

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