ARG65391

anti-CD32 antibody [3D3]

anti-CD32 antibody [3D3] for CyTOF®-candidate,Flow cytometry and Human

Developmental Biology antibody; Immune System antibody

Overview

Product Description Mouse Monoclonal antibody [3D3] recognizes CD32
Tested Reactivity Hu
Tested Application CyTOF®-candidate, FACS
Specificity The clone 3D3 recognizes CD32, a 40 kDa polymorphic transmembrane glycoprotein serving as the low affinity receptor for aggregated IgG. This antibody recognizes CD32 on B cells of all donors, but on platelets, monocytes, and granulocytes of only some donors (131R variant, but not 131H variant).
Host Mouse
Clonality Monoclonal
Clone 3D3
Isotype IgG1
Target Name CD32
Antigen Species Human
Immunogen purified glycosylated recombinant human FcgammaRIIa2
Conjugation Un-conjugated
Alternate Names Fc-gamma RII-a; CD antigen CD32; FcGR; IgG Fc receptor II-a; FCGR2A1; CD32A; FCGR2; Low affinity immunoglobulin gamma Fc region receptor II-a; FcRII-a; Fc-gamma-RIIa; FCG2; IGFR2; CD32; CDw32

Application Instructions

Application Suggestion
Tested Application Dilution
CyTOF®-candidateAssay-dependent
FACS2 - 6 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2212 Human FCGR2A

Swiss-port # P12318 Human Low affinity immunoglobulin gamma Fc region receptor II-a

Gene Symbol FCGR2A
Gene Full Name Fc fragment of IgG, low affinity IIa, receptor (CD32)
Background CD32 (FcgammaRII) is a low affinity receptor for aggregated IgG. It is strongly expressed on monocytes, granulocytes, myeloid and myeloblastic cell lines, and weakly on B cells, CD34+ bone marrow cells, and resting and activated platelets. After binding its ligand, CD32 induces IgG-mediated phagocytosis and oxidative burst in monocytes and neutrophils, whereas in B cells it mediates a negative signal. This polymorphic transmembrane glycoprotein is expressed not only in the activating (CD32a) and inhibitory isoform (CD32b), but also in individual variants with differing avidities for IgG subtypes (e.g. the CD32a131R and CD32a131H allotypes).
Function Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody
Calculated MW 35 kDa
PTM Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK.