ARG22890

anti-CD32 antibody [AT10] (low endotoxin)

anti-CD32 antibody [AT10] (low endotoxin) for Flow cytometry,Functional study,IHC-Frozen sections,Immunoprecipitation and Dog,Human,Pig,Rhesus Monkey

Overview

Product Description Azide free and low endotoxin Mouse Monoclonal antibody [AT10] recognizes CD32
Mouse anti Human CD32 antibody, clone AT10 recognizes the human CD32 antigen, a ~40 kDa glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets.Clone AT10 blocks the binding of IgG to Fc gamma RII (Larsson et al. 1997).
Tested Reactivity Hu, Dog, Pig, R. Mk
Tested Application FACS, FuncSt, IHC-Fr, IP
Host Mouse
Clonality Monoclonal
Clone AT10
Isotype IgG1
Target Name CD32
Antigen Species Human
Immunogen K562 cell line.
Conjugation Un-conjugated
Alternate Names Fc-gamma RII-a; CD antigen CD32; FcGR; IgG Fc receptor II-a; FCGR2A1; CD32A; FCGR2; Low affinity immunoglobulin gamma Fc region receptor II-a; FcRII-a; Fc-gamma-RIIa; FCG2; IGFR2; CD32; CDw32

Application Instructions

Application Suggestion
Tested Application Dilution
FACS20 µg/ml
FuncStAssay-dependent
IHC-Fr1:500 - 1:1000
IP20 µg/ml
Application Note Functional study: This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
IHC-Fr: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Arigo recommends the use of acetone fixation for frozen sections.
FACS: Use 10 µl of the suggested working dilution to label 10^6 cells or cells or 100 µl whole blood.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Purification Note Low endotoxin, ARG22890 has an endotoxin level of 10 EU/ml or lower.
Buffer PBS
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2212 Human FCGR2A

Swiss-port # P12318 Human Low affinity immunoglobulin gamma Fc region receptor II-a

Gene Symbol FCGR2A
Gene Full Name Fc fragment of IgG, low affinity IIa, receptor (CD32)
Background This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2008]
Function Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens. [UniProt]
Calculated MW 35 kDa
PTM Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK.