ARG23573

anti-CD32B antibody [AT130-5R]

anti-CD32B antibody [AT130-5R] for IHC-Frozen sections and Mouse

Overview

Product Description Rat Monoclonal antibody [AT130-5R] recognizes CD32B.
Rat anti Mouse CD32B, clone AT130-5R, recognizes mouse CD32B also known as FcRIIB. CD32B (FcRIIB) is a low affinity receptor for IgG1, IgG2a and IgG2b type antibodies. Unlike other FcRs, the intracellular domain of CD32 contains a inhibitory signalling motif (ITIM) that can antagonize signal transduction mediated through other activatory receptors. FcRIIB is expressed on all myeloid populations and on B-cells.
Tested Reactivity Ms
Tested Application IHC-Fr
Host Rat
Clonality Monoclonal
Clone AT130-5R
Isotype IgG2b
Target Name CD32B
Antigen Species Mouse
Immunogen Mouse CD32-Rat CD4 fusion protein.
Conjugation Un-conjugated
Alternate Names Fc-gamma-RIIc; Fc-gamma RII-c; CD32; CD antigen CD32; CD32B; IgG Fc receptor II-c; Low affinity immunoglobulin gamma Fc region receptor II-c; FCG2; IGFR2; FcRII-c; FCGR2; CDw32

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-Fr1:200
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer TRIS buffered saline and 0.09% Sodium azide.
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14130 Mouse FCGR2B

Swiss-port # P08101 Mouse Low affinity immunoglobulin gamma Fc region receptor II

Gene Symbol FCGR2B
Gene Full Name Fc fragment of IgG, low affinity IIb, receptor (CD32)
Background The protein encoded by this gene is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
Function Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. [UniProt]
Calculated MW 36 kDa
PTM Phosphorylated by the SRC-type Tyr-kinases LYN and BLK. [UniProt]