ARG62820

anti-CD34 antibody [4H11(APG)]

anti-CD34 antibody [4H11(APG)] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Pro-B Cell Marker antibody; Endothelial Cell Marker antibody; Angiogenesis Study antibody

Overview

Product Description Mouse Monoclonal antibody [4H11(APG)] recognizes CD34
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-P, WB
Specificity The clone 4H11(APG) reacts with Class III epitope on CD34 (Mucosialin), a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. 4H11(APG) completely blocks binding of Class II antibody QBEnd10 and Class III antibodies BIRMA K3 and 8G12 on KG1a cell line.
HLDA VI; WS Code M MA58
Host Mouse
Clonality Monoclonal
Clone 4H11(APG)
Isotype IgG1
Target Name CD34
Antigen Species Human
Immunogen Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.
Conjugation Un-conjugated
Alternate Names Hematopoietic progenitor cell antigen CD34; CD antigen CD34

Application Instructions

Application Suggestion
Tested Application Dilution
FACS2 µg/ml
ICC/IFAssay-dependent
IHC-P10 µg/ml
WB1 - 2 µg/ml
Application Note WB: Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing condition.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control WB: Positive control: Kg-1a human leukemia cells. Negative control: Jurkat.
IHC-P: Positive control: Placenta endothelium.

Properties

Form Liquid
Purification Purified from ascites by precipitation methods.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 947 Human CD34

Swiss-port # P28906 Human Hematopoietic progenitor cell antigen CD34

Gene Symbol CD34
Gene Full Name CD34 molecule
Background CD34 protein may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
Function CD34 is a possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30306 Pro-B Cell Marker Antibody panel (CD19, CD34, CD38, CD40, CD45) (FACS)
Related products:
CD34 antibodies; CD34 ELISA Kits; CD34 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Pro-B Cell Marker antibody; Endothelial Cell Marker antibody; Angiogenesis Study antibody
Calculated MW 41 kDa
PTM Highly glycosylated.
Phosphorylated on serine residues by PKC.

Clone References

A novel and feasible way to cultivate and purify endothelial progenitor cells from bone marrow of children with congenital heart diseases.

FACS / Human

Wu YT et al.
Chin Med J (Engl).,  (2012)

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5T4 oncofetal antigen is expressed in high risk of relapse childhood pre-B acute lymphoblastic leukemia and is associated with a more invasive and chemotactic phenotype.

FACS / Human

Castro FV et al.
Leukemia.,  (2012)

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Growth factor priming of synovium-derived stem cells for cartilage tissue engineering.

FACS / Cow

Sampat SR et al.
Tissue Eng Part A.,  (2011)

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Small-diameter human vessel wall engineered from bone marrow-derived mesenchymal stem cells (hMSCs).

FACS / Human

Gong Z et al.
FASEB J.,  (2008)

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