ARG65528

anti-CD34 antibody [QBEND/10] (azide free)

anti-CD34 antibody [QBEND/10] (azide free) for Flow cytometry,Functional study,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Primates,Rabbit

Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Pro-B Cell Marker antibody; Endothelial Cell Marker antibody; Angiogenesis Study antibody

Overview

Product Description Azide free Mouse Monoclonal antibody [QBEND/10] recognizes CD34 (Endothelial Cell Marker)
Tested Reactivity Hu, Ms, NHuPrm, Rb
Species Does Not React With Rat, Bov, Dog, Sheep
Tested Application FACS, FuncSt, IHC-Fr, IHC-P, IP, WB
Specificity The antibody recognizes the CD34 antigen, some cross reactions with vascular associated adventitia and some basement membranes. Clone QBEND/10 immunoprecipitates a glycoprotein with a relative MW 110kD expressed on haematopoietic cells and on the established myeloid leukaemic cell line KG1A. The clone QBEND/10 recognises the Class II epitope on CD34.
Host Mouse
Clonality Monoclonal
Clone QBEND/10
Isotype IgG1, kappa
Target Name CD34
Antigen Species Human
Immunogen Human endothelial vesicles
Conjugation Un-conjugated
Alternate Names Hematopoietic progenitor cell antigen CD34; CD antigen CD34

Application Instructions

Application Suggestion
Tested Application Dilution
FACS5 µg/ml
FuncStAssay-dependent
IHC-FrAssay-dependent
IHC-P2 - 8 µg/ml
IPAssay-dependent
WB1 - 2 µg/ml
Application Note Functional Application: The antibody QBEnd-10 induces homotypic adhesion of leukemic cell line.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Purification Note 0.2 µm filter sterilized.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12490 Mouse CD34

GeneID: 947 Human CD34

Swiss-port # P28906 Human Hematopoietic progenitor cell antigen CD34

Swiss-port # Q64314 Mouse Hematopoietic progenitor cell antigen CD34

Gene Symbol CD34
Gene Full Name CD34 molecule
Background CD34 protein may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
Function CD34 is a possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Pro-B Cell Marker antibody; Endothelial Cell Marker antibody; Angiogenesis Study antibody
Calculated MW 41 kDa
PTM Highly glycosylated.
Phosphorylated on serine residues by PKC.

Clone References

c-Myc-mediated repression of miR-15-16 in hypoxia is induced by increased HIF-2α and promotes tumor angiogenesis and metastasis by upregulating FGF2.

Xue G et al.
Oncogene.,  (2015)

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Circulating hematopoietic stem and progenitor cells are myeloid-biased in cancer patients.

Wu WC et al.
Proc Natl Acad Sci U S A.,  (2014)

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Origin of the vasculature supporting growth of primary patient tumor xenografts.

IHC-P, IHC-Fr / Human

Hylander BL et al.
J Transl Med.,  (2013)

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Cyclic tensile stretch modulates osteogenic differentiation of adipose-derived stem cells via the BMP-2 pathway.

ICC/IF / Rat

Yang X et al.
Arch Med Sci.,  (2010)

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Epstein-Barr virus-associated adrenal smooth muscle tumors and disseminated diffuse large B-cell lymphoma in a child with common variable immunodeficiency: a case report and review of the literature.

IHC / Human

Petrilli G et al.
Int J Surg Pathol.,  (2014)

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Genetic predisposition directs breast cancer phenotype by dictating progenitor cell fate.

FACS / Human

Proia TA et al.
Cell Stem Cell.,  (2011)

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Cardiogenol C can induce Mouse Hair Bulge Progenitor Cells to Transdifferentiate into Cardiomyocyte-like Cells.

IHC / Mouse

Yau WW et al.
Proteome Sci.,  (2011)

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Fibronectin promotes the phorbol 12-myristate 13-acetate-induced macrophage differentiation in myeloid leukemia cells.

ICC/IF / Human

Esendagli G et al.
Int J Hematol.,  (2009)

publication_link

 

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Differentiation of rabbit bone marrow mesenchymal stem cells into corneal epithelial cells in vivo and ex vivo.

FACS / Rabbit

Gu S et al.
Mol Vis.,  (2009)

publication_link

 

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Coexistence of different tissue tumourigenesis in an N-methyl-N-nitrosourea-induced mammary carcinoma model: a histopathological report in Sprague-Dawley rats.

IHC / Rat

Esendagli G et al.
Lab Anim.,  (2009)

publication_link

 

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Optimal perfusion of an intact ovary as a prerequisite for successful ovarian cryopreservation.

IHC / Bovine

Gerritse R et al.
Hum Reprod.,  (2008)

publication_link

 

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1,1-Bis(3'-indolyl)-1-(p-biphenyl)methane inhibits basal-like breast cancer growth in athymic nude mice.

ICC/IF / Mouse

Su Y et al.
Breast Cancer Res.,  (2007)

publication_link

 

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Recurrent solitary fibrous tumour in the cerebellopontine angle.

IHC / Dog

Bikmaz K et al.
J Clin Neurosci.,  (2005)

publication_link

 

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