ARG66633

anti-CD35 / CR1 antibody [SQab19151]

anti-CD35 / CR1 antibody [SQab19151] for IHC-Formalin-fixed paraffin-embedded sections and Human

Overview

Product Description Recombinant Rabbit Monoclonal antibody [SQab19151] recognizes CD35 / CR1
Tested Reactivity Hu
Tested Application IHC-P
Host Rabbit
Clonality Monoclonal
Clone SQab19151
Isotype IgG
Target Name CD35 / CR1
Antigen Species Human
Immunogen Synthetic peptide within aa. 1939-2039 of Human CD35.
Conjugation Un-conjugated
Alternate Names C3b/C4b receptor; C4BR; CD antigen CD35; KN; CD35; C3BR; Complement receptor type 1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:200
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C - 25°C) for 30 minutes.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1378 Human CR1

Swiss-port # P17927 Human Complement receptor type 1

Gene Symbol CR1
Gene Full Name complement component (3b/4b) receptor 1 (Knops blood group)
Background This gene is a member of the receptors of complement activation (RCA) family and is located in the 'cluster RCA' region of chromosome 1. The gene encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. The Knops blood group system is a system of antigens located on this protein. The protein mediates cellular binding to particles and immune complexes that have activated complement. Decreases in expression of this protein and/or mutations in its gene have been associated with gallbladder carcinomas, mesangiocapillary glomerulonephritis, systemic lupus erythematosus and sarcoidosis. Mutations in this gene have also been associated with a reduction in Plasmodium falciparum rosetting, conferring protection against severe malaria. Alternate allele-specific splice variants, encoding different isoforms, have been characterized. Additional allele specific isoforms, including a secreted form, have been described but have not been fully characterized. [provided by RefSeq, Jul 2008]
Function Mediates cellular binding of particles and immune complexes that have activated complement. [UniProt]
Cellular Localization Membrane; Single-pass type I membrane protein. [UniProt]
Calculated MW 224 kDa