ARG62827

anti-CD361 / EVI2B antibody [MEM-216]

anti-CD361 / EVI2B antibody [MEM-216] for Flow cytometry,Immunoprecipitation and Human

Cell Biology and Cellular Response antibody

Overview

Product Description

Mouse Monoclonal antibody [MEM-216] recognizes CD361

Tested Reactivity Hu
Tested Application FACS, IP
Specificity The clone MEM-216 recognizes CD361 / EVI2B, almost uncharacterized type I transmembrane protein with broad leukocyte expression, mostly in myeloid and B cells.
HLDA IX.; WS Code 263
Host Mouse
Clonality Monoclonal
Clone MEM-216
Isotype IgG1
Target Name CD361 / EVI2B
Antigen Species Human
Immunogen Raji cells_x000D_
Conjugation Un-conjugated
Alternate Names CD361; EVDB; EVI-2B; CD antigen CD361; Protein EVI2B; Ecotropic viral integration site 2B protein homolog; D17S376

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 4 µg/ml
IPAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control FACS: Positive control: Raji, Daudi, HL-60 and peripheral blood lymphcocytes (strongly positive on CD19+ cells). Negative control: Jurkat and U-937

Properties

Form Liquid
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2124 Human EVI2B

Swiss-port # P34910 Human Protein EVI2B

Gene Symbol EVI2B
Gene Full Name ecotropic viral integration site 2B
Background CD361, also known as EVI2B (Ecotropic Viral Integration site 2B) or EVDB, is a poorly characterized type I transmembrane protein, expressed from one of three genes embedded in intron 27b of the neurofibromatosis type 1 (NF1) gene. The DNA strand that is transcribed to produce CD361 is the complementary one to the strand encoding NF1. Murine homolog to human CD361 is associated with ecotropic viral insertions, which have been implicated in the expression of murine myeloid leukemias. CD361 has been also reported to be involved in melanocyte and keratinocyte differentiation. However, it is expressed mainly in peripheral blood and bone marrow.
Research Area Cell Biology and Cellular Response antibody
Calculated MW 49 kDa