ARG62836

anti-CD4 antibody [GK1.5] (Functional Grade)

anti-CD4 antibody [GK1.5] (Functional Grade) for Flow cytometry,Functional study,ICC/IF,IHC-Frozen sections,Immunoprecipitation and Mouse

Developmental Biology antibody; Immune System antibody; Regulatory T cells Study antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody

Overview

Product Description

Functional Grade Rat Monoclonal antibody [GK1.5] recognizes CD4

Tested Reactivity Ms
Tested Application FACS, FuncSt, ICC/IF, IHC-Fr, IP
Specificity The clone GK1.5 reacts with an extracellular epitope of mouse CD4 transmembrane glycoprotein (55 kDa).
Host Rat
Clonality Monoclonal
Clone GK1.5
Isotype IgG2b, kappa
Target Name CD4
Antigen Species Mouse
Immunogen Mouse CTL clone V4 cells
Epitope extracellular epitope of mouse CD4
Conjugation Un-conjugated
Alternate Names CD4mut; CD antigen CD4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 µg/10^6 cells
FuncStAssay-dependent
ICC/IF1 - 4 µg/ml
IHC-Fr5 - 10 µg/ml
IP1 - 2 µg / 100 - 500 µg of protein in 1 ml lysate
Application Note Functional studies: Isolation and depletion of CD4+ T cells, blocking of ligand binding to CD4.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Purification Note 0.2 µm filter sterilized.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12504 Mouse CD4

Swiss-port # P06332 Mouse T-cell surface glycoprotein CD4

Gene Symbol CD4
Gene Full Name CD4 molecule
Background CD4 is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]
Function CD4 is an integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages. [UniProt]
Highlight Related products:
CD4 antibodies; CD4 ELISA Kits; CD4 Duos / Panels; Anti-Rat IgG secondary antibodies;
Related news:
New antibody panels and duos for Tumor immune microenvironment
Tumor-Infiltrating Lymphocytes (TILs)
Research Area Developmental Biology antibody; Immune System antibody; Regulatory T cells Study antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody
Calculated MW 51 kDa
PTM Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.

Clone References

Myxoma virus expressing a fusion protein of interleukin-15 (IL15) and IL15 receptor alpha has enhanced antitumor activity.

IHC-Fr / Mouse

Tosic V et al.
PLoS One.,  (2014)

publication_link

 

hr_line

Autologous tumor vaccine modified with recombinant new castle disease virus expressing IL-7 promotes antitumor immune response.

Zhao L et al.
J Immunol.,  (2014)

publication_link

 

hr_line

Supplementation of influenza split vaccines with conserved M2 ectodomains overcomes strain specificity and provides long-term cross protection.

Kim MC et al.
Mol Ther.,  (2014)

publication_link

 

hr_line

A quantitative study of the mechanisms behind thymic atrophy in Gαi2-deficient mice during colitis development.

FACS / Mouse

Elgbratt K et al.
PLoS One.,  (2012)

publication_link

 

hr_line