ARG22943
anti-CD40 antibody [LOB7/6]
anti-CD40 antibody [LOB7/6] for Flow cytometry,IHC-Frozen sections,Immunoprecipitation,IHC-Formalin-fixed paraffin-embedded sections,ELISA and Human,Dog
Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Pro-B Cell Marker antibody; Pre-B Cell Marker antibody
Overview
| Product Description | Mouse Monoclonal antibody [LOB7/6] recognizes CD40 Mouse anti Human CD40 antibody, clone LOB7/6 recognizes the human CD40 cell surface antigen, a 48kDa glycoprotein expressed by B lymphocytes and weakly by some monocytes.CD40 is involved in the process of B cell selection in germinal centres and is vital in T cell-B cell interactions. |
|---|---|
| Tested Reactivity | Hu, Dog |
| Tested Application | ELISA, FACS, IHC-Fr, IHC-P, IP |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | LOB7/6 |
| Isotype | IgG2a |
| Target Name | CD40 |
| Antigen Species | Human |
| Immunogen | CD40 Ig(Fc) fusion protein containing the EC region of Human CD40 and Fc region of Human IgG. |
| Conjugation | Un-conjugated |
| Alternate Names | CDw40; CD antigen CD40; Tumor necrosis factor receptor superfamily member 5; Bp50; CD40L receptor; CDW40; TNFRSF5; p50; B-cell surface antigen CD40 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | Tris-buffered saline and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P25942 Human Tumor necrosis factor receptor superfamily member 5 |
|---|---|
| Gene Symbol | CD40 |
| Gene Full Name | CD40 molecule, TNF receptor superfamily member 5 |
| Background | This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014] |
| Function | Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion. [UniProt] |
| Highlight | Related products: CD40 antibodies; CD40 ELISA Kits; CD40 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Lymphoma |
| Research Area | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Pro-B Cell Marker antibody; Pre-B Cell Marker antibody |
| Calculated MW | 31 kDa |
