ARG62843
anti-CD41-CD61 Complex antibody [IVA30]
anti-CD41-CD61 Complex antibody [IVA30] for ELISA,Flow cytometry,Immunoprecipitation,ICC/IF and Bovine
Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [IVA30] recognizes CD41-CD61 Complex |
|---|---|
| Tested Reactivity | Bov |
| Tested Application | ELISA, FACS, ICC/IF, IP |
| Specificity | The clone IVA30 reacts with CD41/CD61 complex (bovine), one of the earliest markers of the megakaryocytic lineage. CD41/CD61 complex acts as the receptor for fibrinogen, fibronectin, Von Willebrand factor, vitronectin, thrombin and mediates platelets aggregation. CD41/CD61 is expressed only by platelets and megakaryocytes; the complex may be absent or strongly reduced in Glanzmann's thrombasthenia (GT). |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | IVA30 |
| Isotype | IgG2 |
| Target Name | CD41-CD61 Complex |
| Antigen Species | Bovine |
| Immunogen | Bovine trombocytes. |
| Conjugation | Un-conjugated |
| Alternate Names | GT; CD antigen CD61; CD61; BDPLT2; GPIIIa; BDPLT16; GP3A; Platelet membrane glycoprotein IIIa; Integrin beta-3 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | CD41 is a transmembrane glycoprotein (integrin family) composed of two chains GPIIb alpha (heavy chain; 120 kDa) and GPIIb beta (light chain; 23 kDa). CD61 non-covalently associates with CD41. |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody |
| Calculated MW | 87 kDa |
| PTM | Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding. |
Clone References
Nomenclature and characterization of leukocyte differentiation antigens in ruminants.
Immunohistochemical reactivity of anti-platelet monoclonal antibodies.
Biochemical characterization of antigens detected with anti-platelet monoclonal antibodies.
Ruminant cluster CD41/CD61.
Proceedings of the 3rd workshop on ruminant leukocyte antigens. Davis, California, 16 July 1995.
