ARG62843

anti-CD41-CD61 Complex antibody [IVA30]

anti-CD41-CD61 Complex antibody [IVA30] for ELISA,Flow cytometry,Immunoprecipitation,ICC/IF and Bovine

Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [IVA30] recognizes CD41-CD61 Complex
Tested Reactivity Bov
Tested Application ELISA, FACS, ICC/IF, IP
Specificity The clone IVA30 reacts with CD41/CD61 complex (bovine), one of the earliest markers of the megakaryocytic lineage. CD41/CD61 complex acts as the receptor for fibrinogen, fibronectin, Von Willebrand factor, vitronectin, thrombin and mediates platelets aggregation. CD41/CD61 is expressed only by platelets and megakaryocytes; the complex may be absent or strongly reduced in Glanzmann's thrombasthenia (GT).
Host Mouse
Clonality Monoclonal
Clone IVA30
Isotype IgG2
Target Name CD41-CD61 Complex
Antigen Species Bovine
Immunogen Bovine trombocytes.
Conjugation Un-conjugated
Alternate Names GT; CD antigen CD61; CD61; BDPLT2; GPIIIa; BDPLT16; GP3A; Platelet membrane glycoprotein IIIa; Integrin beta-3

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACS1 - 4 µg/ml
ICC/IFAssay-dependent
IPAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 515011 Bovine ITGB3

Background CD41 is a transmembrane glycoprotein (integrin family) composed of two chains GPIIb alpha (heavy chain; 120 kDa) and GPIIb beta (light chain; 23 kDa). CD61 non-covalently associates with CD41.
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
Calculated MW 87 kDa
PTM Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding.

Clone References

Nomenclature and characterization of leukocyte differentiation antigens in ruminants.

Naessens J et al.
Immunol Today.,  (1997)

publication_link

 

hr_line

Immunohistochemical reactivity of anti-platelet monoclonal antibodies.

Simon M et al.
Vet Immunol Immunopathol.,  (1996)

publication_link

 

hr_line

Biochemical characterization of antigens detected with anti-platelet monoclonal antibodies.

Mateo A et al.
Vet Immunol Immunopathol.,  (1996)

publication_link

 

hr_line

Ruminant cluster CD41/CD61.

Mateo A et al.
Vet Immunol Immunopathol.,  (1996)

publication_link

 

hr_line

Proceedings of the 3rd workshop on ruminant leukocyte antigens. Davis, California, 16 July 1995.

[No authors listed] et al.
Vet Immunol Immunopathol.,  (1996)

publication_link

 

hr_line