ARG57571

anti-CD41-CD61 Complex antibody [PAC-1]

anti-CD41-CD61 Complex antibody [PAC-1] for Flow cytometry,Immunoprecipitation,ICC/IF and Human

Overview

Product Description Mouse Monoclonal antibody [PAC-1] recognizes CD41-CD61 Complex
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IP
Specificity The antibody recognizes an activation-induced conformational epitope PAC-1 on CD41/CD61 complex (gpIIb/IIIa), also known as integrin alpha IIb beta 3, a receptor which mediates platelet aggregation.
Host Mouse
Clonality Monoclonal
Clone PAC-1
Isotype IgM
Target Name CD41-CD61 Complex
Antigen Species Human
Immunogen Human platelets.
Conjugation Un-conjugated
Alternate Names GTA; GT; GPalpha IIb; PPP1R93; CD41; BDPLT2; BDPLT16; GP2B; Integrin alpha-IIb; GPIIb; Platelet membrane glycoprotein IIb; HPA3; CD antigen CD41; CD41B

Application Instructions

Application Suggestion
Tested Application Dilution
FACS2 - 4 µg/ml
ICC/IFAssay-dependent
IPAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification PEG precipitation and hydroxyapatite chromatography.
Purity > 95% (by SDS-PAGE)
Buffer Tris buffered saline (pH 8.0) and 15 mM Sodium azide.
Preservative 15mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3674 Human ITGA2B

Swiss-port # P08514 Human Integrin alpha-IIb

Gene Symbol ITGA2B
Gene Full Name ITGA2B
Background ITGA2B encodes integrin alpha chain 2b. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 2b undergoes post-translational cleavage to yield disulfide-linked light and heavy chains that join with beta 3 to form a fibronectin receptor expressed in platelets that plays a crucial role in coagulation. Mutations that interfere with this role result in thrombasthenia. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq, Jul 2008]
Function Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface. [UniProt]
Calculated MW 113 kDa