ARG62844

anti-CD41 antibody [HIP8]

anti-CD41 antibody [HIP8] for CyTOF®-candidate,Flow cytometry,IHC-Frozen sections and Human,Primates

Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody

Overview

Product Description

Mouse Monoclonal antibody [HIP8] recognizes CD41

Tested Reactivity Hu, NHuPrm
Tested Application CyTOF®-candidate, FACS, IHC-Fr
Specificity The clone HIP8 reacts with alpha (a) subunit of CD41 (heavy chain; 120 kDa). CD41 is mainly expressed on platelets and megakaryocytes.
HIP8 blocks platelet aggregation and completely inhibits ADP-, epinephrine-, and collagen-induced platelet activation, and partially inhibits ristocetin- and thrombin-induced platelet activation. HIP8 is useful in the morphological and physiological studies of platelets and megakaryocytes.
HLDA IV; WS Code P 38
Host Mouse
Clonality Monoclonal
Clone HIP8
Isotype IgG1
Target Name CD41
Conjugation Un-conjugated
Alternate Names GTA; GT; GPalpha IIb; PPP1R93; CD41; BDPLT2; BDPLT16; GP2B; Integrin alpha-IIb; GPIIb; Platelet membrane glycoprotein IIb; HPA3; CD antigen CD41; CD41B

Application Instructions

Application Suggestion
Tested Application Dilution
CyTOF®-candidateAssay-dependent
FACS1 - 4 µg/ml
IHC-FrAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control FACS: Human platelets.

Properties

Form Liquid
Purification Purified by protein A
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3674 Human ITGA2B

Swiss-port # P08514 Human Integrin alpha-IIb

Gene Symbol ITGA2B
Gene Full Name integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41)
Background CD41 (platelet glycoprotein IIb) is composed of two subunits (120 kDa a, alpha and 23 kDa b, beta) that interact with CD61 in the presence of calcium to form a functional adhesive protein receptor. Upon blood vessel damage, this receptor binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin. CD41 is mainly expressed on megakaryocyte-platelet lineage, but generally belongs to the antigens that are expressed during early stages of hematopoietic differentiation.
Function Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface. [UniProt]
Highlight Related products:
CD41 antibodies; Anti-Mouse IgG secondary antibodies;
Related news:
CyTOF-candidate Antibodies
Research Area Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
Calculated MW 113 kDa