ARG65500

anti-CD43 antibody [MEM-59] (low endotoxin)

anti-CD43 antibody [MEM-59] (low endotoxin) for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot,Functional study and Human

Developmental Biology antibody; Immune System antibody

Overview

Product Description Azide free and low endotoxin Mouse Monoclonal antibody [MEM-59] recognizes CD43
Tested Reactivity Hu
Tested Application FACS, FuncSt, IHC-P, IP, WB
Specificity The clone MEM-59 recognizes neuraminidase-sensitive epitope on CD43 (Leukosialin), a 95-135 kDa type I transmembrane glycoprotein (mucin-type) which is involved in lymphocyte activation. CD43 is expressed by platelets and at high levels on the surface of all leukocytes; it is negative on resting B lymphocytes and erythrocytes.
HLDA IV; WS Code NL 604
HLDA V; WS Code AS S290
Host Mouse
Clonality Monoclonal
Clone MEM-59
Isotype IgG1
Target Name CD43
Antigen Species Human
Immunogen Human T lymphocytes.
Conjugation Un-conjugated
Alternate Names CD antigen CD43; B-cell differentiation antigen LP-3; Sialophorin; Cd43; Galgp; A630014B01Rik; Ly-48; Lymphocyte antigen 48; Ly48; Leukocyte sialoglycoprotein; Leukosialin

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 µg/ml
FuncStAssay-dependent
IHC-P10 µg/ml
IPAssay-dependent
WBAssay-dependent
Application Note Functional studies: The clone MEM-59 activates T lymphocytes and also induces apoptosis in hemopoietic progenitor cells.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control IHC-P: Spleen, thymus and tonsil.

Properties

Form Liquid
Purification Purification with Protein A.
Purification Note 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6693 Human SPN

Swiss-port # P16150 Human Leukosialin

Gene Symbol SPN
Gene Full Name sialophorin
Background CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumour suppressor p53 protein, but in case of p53 and ARF defficiency CD43 promotes tumour proliferation and viability. It appears to be an important modulator of leukocyte functions.
Function One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response (By similarity). [UniProt]
Research Area Developmental Biology antibody; Immune System antibody
Calculated MW 40 kDa

Clone References

CD24 expression identifies teratogen-sensitive fetal neural stem cell subpopulations: evidence from developmental ethanol exposure and orthotopic cell transfer models.

FACS / Mouse

Tingling JD et al.
PLoS One.,  (2013)

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The carboxy terminus of NBS1 is required for induction of apoptosis by the MRE11 complex.

WB / Mouse

Stracker TH et al.
Nature.,  (2007)

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Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules.

Cermák L et al.
J Biol Chem.,  (2002)

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