ARG62852

anti-CD44 antibody [MEM-263]

anti-CD44 antibody [MEM-263] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Dog,Human,Pig

Cancer antibody; Developmental Biology antibody; Immune System antibody; Chondrogenesis Study antibody

Overview

Product Description

Mouse Monoclonal antibody [MEM-263] recognizes CD44

Tested Reactivity Hu, Dog, Pig
Predict Reactivity Mk
Tested Application FACS, IHC-P, IP, WB
Specificity The clone MEM-263 reacts with extracellular (N-terminal) domain of standard CD44 (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes.
HLDA III; WS Code T 155
Host Mouse
Clonality Monoclonal
Clone MEM-263
Isotype IgG1
Target Name CD44
Immunogen COS-7 cells (African Green Monkey).
Conjugation Un-conjugated
Alternate Names MDU2; MDU3; GP90 lymphocyte homing/adhesion receptor; Hermes antigen; Extracellular matrix receptor III; PGP-I; Epican; CDW44; Phagocytic glycoprotein 1; Pgp1; HUTCH-I; MC56; Hyaluronate receptor; CD antigen CD44; Heparan sulfate proteoglycan; CD44 antigen; LHR; IN; HCELL; Phagocytic glycoprotein I; PGP-1; CSPG8; MIC4; ECMR-III; CDw44

Application Instructions

Application Suggestion
Tested Application Dilution
FACS4 µg/ml
IHC-P10 µg/ml
IPAssay-dependent
WB2 µg/ml
Application Note WB: 2 μg/ml dilution, 60 min on vertical incubator Positive control: Kg-1a human acute leukemia cell lysate JURKAT human leukemia T-cell lysate Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (6% separating gel).
IHC-P: Positive tissue: uterus, myometrium
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 960 Human CD44

Swiss-port # P16070 Human CD44 antigen

Background CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.
Highlight Related Antibody Duos and Panels:
ARG30314 Chondrogenesis Marker Antibody Panel
Related products:
CD44 antibodies; CD44 ELISA Kits; CD44 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Research Area Cancer antibody; Developmental Biology antibody; Immune System antibody; Chondrogenesis Study antibody
Calculated MW 82 kDa
PTM Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N- and O-glycosylated. O-glycosylation contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s). It is uncertain if O-glycosylation occurs on Thr-637 or Thr-638.
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.

Clone References

Repeated autologous bone marrow-derived mesenchymal stem cell injections improve radiation-induced proctitis in pigs.

Linard C et al.
Stem Cells Transl Med.,  (2013)

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Loss of the malignant phenotype of human neuroblastoma cells by a catalytically inactive dominant-negative hTERT mutant.

Samy M et al.
Mol Cancer Ther.,  (2012)

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Alternative pathway activation of complement by Shiga toxin promotes exuberant C3a formation that triggers microvascular thrombosis.

BL / Human

Morigi M et al.
J Immunol.,  (2011)

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