ARG62856
anti-CD45 antibody [MEM-28]
anti-CD45 antibody [MEM-28] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody; Mouse Inflammatory Cell Marker antibody; B Cell Marker antibody
Overview
| Product Description | Mouse Monoclonal antibody [MEM-28] recognizes CD45 |
|---|---|
| Tested Reactivity | Hu |
| Species Does Not React With | Hrs |
| Tested Application | FACS, ICC/IF, IHC-P, IP, WB |
| Specificity | The clone MEM-28 reacts with all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets. HLDA III; WS Code NL 833a |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-28 |
| Isotype | IgG1 |
| Target Name | CD45 |
| Antigen Species | Human |
| Immunogen | Human thymocytes and T lymphocytes. |
| Conjugation | Un-conjugated |
| Alternate Names | LY5; GP180; Receptor-type tyrosine-protein phosphatase C; CD45; L-CA; CD antigen CD45; Leukocyte common antigen; CD45R; LCA; T200; EC 3.1.3.48; B220 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Positive control: JURKAT human leukemia T-cell lysateKg-1a human leukemia cell lysate Sample preparation: buffer with laurylmaltoside, 2 x non-reducing SDS Application note: Non-reducing conditions. SDS-PAGE (6% separating gel). Immunohistochemistry (paraffin sections): No pre-treatment of tissue sections is essential. Immunocytochemistry: Paraformaldehyde fixation can be used. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by protein A |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P08575 Human Receptor-type tyrosine-protein phosphatase C |
|---|---|
| Gene Symbol | PTPRC |
| Gene Full Name | protein tyrosine phosphatase, receptor type, C |
| Background | CD45 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012] |
| Function | CD45: Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30306 Pro-B Cell Marker Antibody panel (CD19, CD34, CD38, CD40, CD45) (FACS) ARG30307 Pre-B Cell Marker Antibody Panel (CD19, CD25, CD38, CD40, CD45) (FACS) Related products: CD45 antibodies; CD45 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Lymphoma |
| Research Area | Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody; Mouse Inflammatory Cell Marker antibody; B Cell Marker antibody |
| Calculated MW | 147 kDa |
| PTM | Heavily N- and O-glycosylated. |
Customer's Feedback
Average
Review for anti-CD45 antibody [MEM-28]
Application：WB
Sample：Jurkat
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Clone References
Adult human glia, pericytes and meningeal fibroblasts respond similarly to IFNy but not to TGFβ1 or M-CSF.
ICC/IF / Human
Individual profiling of circulating tumor cell composition and therapeutic outcome in patients with hepatocellular carcinoma.
ICC/IF / Human
M-CSF increases proliferation and phagocytosis while modulating receptor and transcription factor expression in adult human microglia.
ICC/IF /
Microparticles (ectosomes) shed by stored human platelets downregulate macrophages and modify the development of dendritic cells.
FACS / Human
HIV Type 1 Nef is released from infected cells in CD45(+) microvesicles and is present in the plasma of HIV-infected individuals.
WB / Human
