ARG62856

anti-CD45 antibody [MEM-28]

anti-CD45 antibody [MEM-28] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human

Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody; Mouse Inflammatory Cell Marker antibody; B Cell Marker antibody
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Overview

Product Description

Mouse Monoclonal antibody [MEM-28] recognizes CD45

Tested Reactivity Hu
Species Does Not React With Hrs
Tested Application FACS, ICC/IF, IHC-P, IP, WB
Specificity The clone MEM-28 reacts with all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.
HLDA III; WS Code NL 833a
Host Mouse
Clonality Monoclonal
Clone MEM-28
Isotype IgG1
Target Name CD45
Antigen Species Human
Immunogen Human thymocytes and T lymphocytes.
Conjugation Un-conjugated
Alternate Names LY5; GP180; Receptor-type tyrosine-protein phosphatase C; CD45; L-CA; CD antigen CD45; Leukocyte common antigen; CD45R; LCA; T200; EC 3.1.3.48; B220

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 µg/ml
ICC/IF10 µg/ml
IHC-P10 µg/ml
IPAssay-dependent
WB1 µg/ml
Application Note WB: Positive control: JURKAT human leukemia T-cell lysateKg-1a human leukemia cell lysate Sample preparation: buffer with laurylmaltoside, 2 x non-reducing SDS Application note: Non-reducing conditions. SDS-PAGE (6% separating gel).
Immunohistochemistry (paraffin sections): No pre-treatment of tissue sections is essential.
Immunocytochemistry: Paraformaldehyde fixation can be used.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified by protein A
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5788 Human PTPRC

Swiss-port # P08575 Human Receptor-type tyrosine-protein phosphatase C

Gene Symbol PTPRC
Gene Full Name protein tyrosine phosphatase, receptor type, C
Background CD45 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]
Function CD45: Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.

(Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30306 Pro-B Cell Marker Antibody panel (CD19, CD34, CD38, CD40, CD45) (FACS)
ARG30307 Pre-B Cell Marker Antibody Panel (CD19, CD25, CD38, CD40, CD45) (FACS)
Related products:
CD45 antibodies; CD45 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
Lymphoma
Research Area Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody; Mouse Inflammatory Cell Marker antibody; B Cell Marker antibody
Calculated MW 147 kDa
PTM Heavily N- and O-glycosylated.

Customer's Feedback

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Review for anti-CD45 antibody [MEM-28]

Application:WB

Sample:Jurkat

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Clone References

Adult human glia, pericytes and meningeal fibroblasts respond similarly to IFNy but not to TGFβ1 or M-CSF.

ICC/IF / Human

Smith AM et al.
PLoS One.,  (2013)

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Individual profiling of circulating tumor cell composition and therapeutic outcome in patients with hepatocellular carcinoma.

ICC/IF / Human

Nel I et al.
Transl Oncol.,  (2013)

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M-CSF increases proliferation and phagocytosis while modulating receptor and transcription factor expression in adult human microglia.

ICC/IF / 

Smith AM et al.
J Neuroinflammation.,  (2013)

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Microparticles (ectosomes) shed by stored human platelets downregulate macrophages and modify the development of dendritic cells.

FACS / Human

Sadallah S et al.
J Immunol.,  (2011)

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HIV Type 1 Nef is released from infected cells in CD45(+) microvesicles and is present in the plasma of HIV-infected individuals.

WB / Human

Raymond AD et al.
AIDS Res Hum Retroviruses.,  (2011)

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