ARG62418
anti-CD47 antibody [B6H12.2]
anti-CD47 antibody [B6H12.2] for Flow cytometry,Functional study,ICC/IF,IHC-Frozen sections,Immunoprecipitation,Neutralizing,Western blot and Human
Cancer antibody; Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [B6H12.2] recognizes CD47 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, FuncSt, ICC/IF, IHC-Fr, IP, Neut, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | B6H12.2 |
| Isotype | IgG1, kappa |
| Target Name | CD47 |
| Antigen Species | Human |
| Immunogen | Intact CD47 purified from placenta. |
| Epitope | Ig domain |
| Conjugation | Un-conjugated |
| Alternate Names | Leukocyte surface antigen CD47; CD antigen CD47; Antigenic surface determinant protein OA3; MER6; OA3; Protein MER6; IAP; Integrin-associated protein |
Application Instructions
| Application Suggestion |
|
||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | Functional study, Neutralizing: This antibody blocks the binding of SIRP alpha (Yoshida, et al.; Latour et al.) and inhibits in assays where CD47-integrin association is required.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||||||||||||
| Positive Control | Tonsil. |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein G purified |
| Buffer | 10mM PBS (pH 7.4), 0.2% BSA and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 0.2% BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD47 |
| Gene Full Name | CD47 molecule |
| Background | This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010] |
| Function | Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. [UniProt] |
| Cellular Localization | Cell membrane |
| Research Area | Cancer antibody; Immune System antibody |
| Calculated MW | 35 kDa |
Clone References
CD47-dependent immunomodulatory and angiogenic activities of extracellular vesicles produced by T cells.
WB / Cow
The CD47-signal regulatory protein alpha (SIRPa) interaction is a therapeutic target for human solid tumors.
IHC-Fr / Human
H-ras up-regulates expression of BNIP3.
FACS / Human, Mouse
Heparan sulfate modification of the transmembrane receptor CD47 is necessary for inhibition of T cell receptor signaling by thrombospondin-1.
WB, IP / Human
Thrombospondin-1 inhibits VEGF receptor-2 signaling by disrupting its association with CD47.
ICC/IF / Human
Increasing survival of ischemic tissue by targeting CD47.
WB / Mouse
Increased erythrocyte adhesion in mice and humans with hereditary spherocytosis and hereditary elliptocytosis.
BL / Human
