ARG23111
anti-CD47 antibody [BRIC126]
anti-CD47 antibody [BRIC126] for IHC-Frozen sections,Flow cytometry,Functional study,Immunoprecipitation,Western blot and Human,Dog,Bovine,Sheep,Pig
Overview
| Product Description | Mouse Monoclonal antibody [BRIC126] recognizes CD47 Mouse anti human CD47 antibody, clone BRIC126 recognises the human CD47 cell surface glycoprotein, a heavily N-glycosylated 47-52 kDa molecule. CD47 is expressed on all cells and tissues so far examined, although expression is reduced on erythrocytes of the rare Rh null phenotype. |
|---|---|
| Tested Reactivity | Hu, Bov, Dog, Pig, Sheep |
| Tested Application | FACS, FuncSt, IHC-Fr, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | BRIC126 |
| Isotype | IgG2b |
| Target Name | CD47 |
| Antigen Species | Human |
| Immunogen | Human erythrocytes |
| Conjugation | Un-conjugated |
| Alternate Names | Leukocyte surface antigen CD47; CD antigen CD47; Antigenic surface determinant protein OA3; MER6; OA3; Protein MER6; IAP; Integrin-associated protein |
Application Instructions
| Application Suggestion |
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| Application Note | Functional study: This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. WB: This product recognizes CD47 under non-reducing conditions, the CD47 epitope recognized is lost on reduction. FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | Tris-buffered saline and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD47 |
| Gene Full Name | CD47 molecule |
| Background | This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010] |
| Function | Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. [UniProt] |
| Calculated MW | 35 kDa |
