ARG62871

anti-CD47 antibody [MEM-122]

anti-CD47 antibody [MEM-122] for Flow cytometry,IHC-Frozen sections,Western blot and Human,Pig,Primates

Cancer antibody; Immune System antibody

Overview

Product Description

Mouse Monoclonal antibody [MEM-122] recognizes CD47

Tested Reactivity Hu, NHuPrm, Pig
Tested Application FACS, IHC-Fr, WB
Specificity The clone MEM-122 reacts with an extracellular epitope of CD47 (Integrin Associated Protein), a 50-55 kDa membrane adhesion molecule (thrombospondin receptor; immunoglobulin supergene family) expressed on leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines.
HLDA VI; WS Code AS A051
Host Mouse
Clonality Monoclonal
Clone MEM-122
Isotype IgM
Target Name CD47
Immunogen COS-7 (African green monkey) cells
Conjugation Un-conjugated
Alternate Names Leukocyte surface antigen CD47; CD antigen CD47; Antigenic surface determinant protein OA3; MER6; OA3; Protein MER6; IAP; Integrin-associated protein

Application Instructions

Application Suggestion
Tested Application Dilution
FACS2 µg/ml
IHC-FrAssay-dependent
WBAssay-dependent
Application Note WB: Under non-reducing condition.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from ascites by CHT-chromatography and precipitation methods.
Purity > 95% (by SDS-PAGE)
Buffer TBS (pH 8.0) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 397042 Pig CD47

GeneID: 961 Human CD47

Swiss-port # Q08722 Human Leukocyte surface antigen CD47

Swiss-port # Q9GKE8 Pig Leukocyte surface antigen CD47

Gene Symbol CD47
Gene Full Name CD47 molecule
Background CD47 (integrin-associated protein, IAP) is an ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Binding secreted thrombospondin-1, CD47 counteracts graft vascularization. CD47 acts also as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion and T cell activation. CD47 is also involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.
Function Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. [UniProt]
Research Area Cancer antibody; Immune System antibody
Calculated MW 35 kDa