ARG23695

anti-CD49b / Integrin alpha 2 antibody [AK7]

anti-CD49b / Integrin alpha 2 antibody [AK7] for Flow cytometry,Functional study,IHC-Frozen sections and Human,Rhesus Monkey,Baboon

Overview

Product Description Mouse Monoclonal antibody [AK7] recognizes CD49b / Integrin alpha 2.
Clone AK7 recognizes the integrin alpha 2 subunit, a ~160 kDa glycoprotein that non-covalently associates with the ~130 kDa integrin beta 1 subunit to form the VLA-2 complex. CD49b is expressed by platelets, long term cultivated T cells, approximately 50% of monocytes and most adherent cell lines. Mouse anti Human CD49b antibody, clone AK7 inhibits cell attachment to collagen.
Tested Reactivity Hu, Bb, R. Mk
Tested Application FACS, FuncSt, IHC-Fr
Host Mouse
Clonality Monoclonal
Clone AK7
Isotype IgG1
Target Name CD49b / Integrin alpha 2
Antigen Species Human
Conjugation Un-conjugated
Alternate Names Collagen receptor; VLA-2 subunit alpha; HPA-5; CD49B; CD49 antigen-like family member B; GPIa; VLA-2; CD antigen CD49b; BR; VLAA2; Platelet membrane glycoprotein Ia; Integrin alpha-2

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:50 - 1:100
FuncStAssay-dependent
IHC-Fr1:500 - 1:1000
Application Note IHC-Fr: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Arigo recommends the use of acetone fixation for frozen sections.
FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3673 Human ITGA2

Swiss-port # P17301 Human Integrin alpha-2

Gene Symbol ITGA2
Gene Full Name integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)
Background This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
Function Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. [UniProt]
Calculated MW 129 kDa