ARG23698
anti-CD49b / Integrin alpha 2 antibody [AK7] (FITC)
anti-CD49b / Integrin alpha 2 antibody [AK7] (FITC) for Flow cytometry and Human,Rhesus Monkey,Baboon
Overview
Product Description | FITC-conjugated Mouse Monoclonal antibody [AK7] recognizes CD49b / Integrin alpha 2. Clone AK7 recognizes the integrin alpha 2 subunit, a ~160 kDa glycoprotein that non-covalently associates with the ~130 kDa integrin beta 1 subunit to form the VLA-2 complex. CD49b is expressed by platelets, long term cultivated T cells, approximately 50% of monocytes and most adherent cell lines. Mouse anti Human CD49b antibody, clone AK7 inhibits cell attachment to collagen. |
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Tested Reactivity | Hu, Bb, R. Mk |
Tested Application | FACS |
Host | Mouse |
Clonality | Monoclonal |
Clone | AK7 |
Isotype | IgG1 |
Target Name | CD49b / Integrin alpha 2 |
Antigen Species | Human |
Conjugation | FITC |
Alternate Names | Collagen receptor; VLA-2 subunit alpha; HPA-5; CD49B; CD49 antigen-like family member B; GPIa; VLA-2; CD antigen CD49b; BR; VLAA2; Platelet membrane glycoprotein Ia; Integrin alpha-2 |
Application Instructions
Application Suggestion |
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Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells or 100 µl whole blood. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
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Purification | Purification with Protein A. |
Buffer | PBS, 0.09% Sodium azide and 1% BSA |
Preservative | 0.09% Sodium azide |
Stabilizer | 1% BSA |
Concentration | 0.1 mg/ml |
Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Gene Symbol | ITGA2 |
Gene Full Name | integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor) |
Background | This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] |
Function | Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. [UniProt] |
Calculated MW | 129 kDa |