ARG57601

anti-CD49b / Integrin alpha 2 antibody

anti-CD49b / Integrin alpha 2 antibody for Flow cytometry,ICC/IF,Immunohistochemistry,Immunoprecipitation,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes CD49b / Integrin alpha 2
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CD49b / Integrin alpha 2
Antigen Species Human
Immunogen Synthetic peptide derived from Human Integrin alpha 2.
Conjugation Un-conjugated
Alternate Names Collagen receptor; VLA-2 subunit alpha; HPA-5; CD49B; CD49 antigen-like family member B; GPIa; VLA-2; CD antigen CD49b; BR; VLAA2; Platelet membrane glycoprotein Ia; Integrin alpha-2

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:100
ICC/IF1:50 - 1:200
IHC1:50 - 1:200
IP1:100
WB1:1000 - 1:2000
Application Note IHC-P: Antigen Retrieval: Microwave (8 min, twice) tissue section in Sodium citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16398 Mouse ITGA2

GeneID: 3673 Human ITGA2

Swiss-port # P17301 Human Integrin alpha-2

Swiss-port # Q62469 Mouse Integrin alpha-2

Gene Symbol ITGA2
Gene Full Name integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)
Background This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
Function Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. [UniProt]
Cellular Localization Cytoplasm
Calculated MW 129 kDa