ARG23660
anti-CD49c / Integrin alpha 3 antibody [P1B5]
anti-CD49c / Integrin alpha 3 antibody [P1B5] for Flow cytometry,ICC/IF,Immunoprecipitation,IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
| Product Description | Mouse Monoclonal antibody [P1B5] recognizes CD49c / Integrin alpha 3. This product recognizes CD49c, a 150 kDa cell surface antigen which is also known as the alpha-3 integrin and as VLA-3. CD49c is very weakly expressed on peripheral blood leucocytes and is not found on platelets. Epithelial cells and endothelial cells express significant levels of CD49c. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-P, IP |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | P1B5 |
| Isotype | IgG1 |
| Target Name | CD49c / Integrin alpha 3 |
| Antigen Species | Human |
| Immunogen | Non-trypsinized HT-1080 cells. |
| Conjugation | Un-conjugated |
| Alternate Names | FRP-2; MSK18; GAPB3; CD49 antigen-like family member C; CD49C; ILNEB; Integrin alpha-3; VL3A; Galactoprotein B3; VLA3a; CD antigen CD49c; VCA-2; VLA-3 subunit alpha; GAP-B3 |
Application Instructions
| Application Suggestion |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ITGA3 |
| Gene Full Name | integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor) |
| Background | The protein encoded by this gene belongs to the family of integrins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain, and function as cell surface adhesion molecules. This gene encodes alpha 3 subunit, which undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 subunit to form an integrin that interacts with many extracellular-matrix proteins. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Oct 2008] |
| Function | Integrin alpha-3/beta-1 is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. [UniProt] |
| Calculated MW | 117 kDa |
| PTM | Isoform 1, but not isoform 2, is phosphorylated on serine residues. Phosphorylation increases after phorbol 12-myristate 13-acetate stimulation. [UniProt] |
