ARG23116
anti-CD49d / LPAM1 / Integrin alpha 4 antibody [44H6]
anti-CD49d / LPAM1 / Integrin alpha 4 antibody [44H6] for IHC-Frozen sections,Flow cytometry and Human
Overview
| Product Description | Mouse Monoclonal antibody [44H6] recognizes CD49d / LPAM1 / Integrin alpha 4 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-Fr |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 44H6 |
| Isotype | IgG1 |
| Target Name | CD49d / LPAM1 / Integrin alpha 4 |
| Antigen Species | Human |
| Immunogen | HOON pre-B leukaemia cell line. |
| Conjugation | Un-conjugated |
| Alternate Names | CD49 antigen-like family member D; VLA-4 subunit alpha; CD49D; Integrin alpha-IV; CD antigen CD49d; Integrin alpha-4; IA4 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-Fr: Overnight incubation with clone 44H6 is recommended for optimal results. The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Arigo recommends the use of acetone fixation for frozen sections. FACS: Use 10 µl of the suggested working dilution to label 100 µl of human whole peripheral blood. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by ion exchange chromatography. |
| Buffer | PBS and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ITGA4 |
| Gene Full Name | integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor) |
| Background | The product of this gene belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an alpha 4 chain. Unlike other integrin alpha chains, alpha 4 neither contains an I-domain, nor undergoes disulfide-linked cleavage. Alpha 4 chain associates with either beta 1 chain or beta 7 chain. [provided by RefSeq, Jul 2008] |
| Function | Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. [UniProt] |
| Calculated MW | 115 kDa |
| PTM | Phosphorylation on Ser-1027 inhibits PXN binding. |
