ARG42288
anti-CD49d / LPAM1 / Integrin alpha 4 antibody [9F10] (low endotoxin)
anti-CD49d / LPAM1 / Integrin alpha 4 antibody [9F10] (low endotoxin) for Flow cytometry,IHC-Frozen sections,Functional study and Sheep,Cat,Horse,Dog,Cow,Primates,Human
Overview
| Product Description | Azide free and low endotoxin Mouse Monoclonal antibody [9F10] recognizes CD49d / LPAM1 / Integrin alpha 4 |
|---|---|
| Tested Reactivity | Hu, Cat, Cow, Dog, Hrs, NHuPrm, Sheep |
| Tested Application | FACS, FuncSt, IHC-Fr |
| Specificity | The mouse monoclonal antibody 9F10 recognizes an extracellular epitope of CD49d (alpha 4 integrin), a 145-180 kDa type I transmembrane glycoprotein expressed on B and T cells, monocytes, eosinophils, basophils, NK cells, and dendritic cells, but not platelets. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 9F10 |
| Isotype | IgG1, kappa |
| Target Name | CD49d / LPAM1 / Integrin alpha 4 |
| Antigen Species | Human |
| Immunogen | Human CD49d. |
| Conjugation | Un-conjugated |
| Alternate Names | CD49 antigen-like family member D; VLA-4 subunit alpha; CD49D; Integrin alpha-IV; CD antigen CD49d; Integrin alpha-4; IA4 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Purification Note | 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein. |
| Buffer | PBS |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ITGA4 |
| Gene Full Name | integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor) |
| Background | The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 4 subunit. This subunit associates with a beta 1 or beta 7 subunit to form an integrin that may play a role in cell motility and migration. This integrin is a therapeutic target for the treatment of multiple sclerosis, Crohn's disease and inflammatory bowel disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015] |
| Function | Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415). ITGA4:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). [UniProt] |
| Cellular Localization | Membrane; Single-pass type I membrane protein. [UniProt] |
| Calculated MW | 115 kDa |
| PTM | Phosphorylation on Ser-1027 inhibits PXN binding. [UniProt] |
