ARG57602

anti-CD49e / Integrin alpha 5 antibody

anti-CD49e / Integrin alpha 5 antibody for Flow cytometry,ICC/IF,Immunohistochemistry,Immunoprecipitation,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes CD49e / Integrin alpha 5
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CD49e / Integrin alpha 5
Antigen Species Human
Immunogen Synthetic peptide derived from Human Integrin alpha 5.
Conjugation Un-conjugated
Alternate Names CD49e; CD49 antigen-like family member E; Integrin alpha-F; Fibronectin receptor subunit alpha; CD antigen CD49e; VLA-5; FNRA; VLA5A; Integrin alpha-5

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
ICC/IF1:50 - 1:200
IHC1:50 - 1:200
IPAssay-dependent
WB1:1000 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16402 Mouse ITGA5

GeneID: 3678 Human ITGA5

Swiss-port # P08648 Human Integrin alpha-5

Swiss-port # P11688 Mouse Integrin alpha-5

Gene Symbol ITGA5
Gene Full Name integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
Background The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. Integrin alpha 5 and integrin alpha V chains are produced by distinct genes. [provided by RefSeq, Jan 2015]
Function Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. [UniProt]
Calculated MW 115 kDa
PTM Proteolytic cleavage by PCSK5 mediates activation of the precursor. [UniProt]