ARG23224

anti-CD49f / Integrin alpha 6 antibody [450-30A]

anti-CD49f / Integrin alpha 6 antibody [450-30A] for IHC-Frozen sections,Flow cytometry,Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [450-30A] recognizes CD49f / Integrin alpha 6
Mouse anti Human CD49f antibody, clone 450-30A recognizes the human VLA-6 cell surface antigen, also known as the alpha 6 integrin and as CD49f. CD49f is expressed by platelets, weakly by monocytes and by a subset of lymphocytes. CD49f is also widely expressed on epithelial tissues.
Tested Reactivity Hu
Tested Application FACS, IHC-Fr, WB
Host Mouse
Clonality Monoclonal
Clone 450-30A
Isotype IgG1
Target Name CD49f / Integrin alpha 6
Antigen Species Human
Immunogen Alpha 6 beta 4 integrin purified from A431 cells.
Conjugation Un-conjugated
Alternate Names CD49 antigen-like family member F; ITGA6B; Alpha6p; VLA-6; CD antigen CD49f; CD49f; Integrin alpha-6

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:50
IHC-Fr1:50 - 1:100
WBAssay-dependent
Application Note IHC-Fr: The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Arigo recommends the use of acetone fixation for frozen sections.
FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% Sodium azide.
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3655 Human ITGA6

Swiss-port # P23229 Human Integrin alpha-6

Gene Symbol ITGA6
Gene Full Name integrin, alpha 6
Background The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome. [UniProt]
Calculated MW 127 kDa
PTM Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand.

In invasive prostate cancer ITGA6 undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner enhancing cell invasion and migration in vitro.

Palmitoylation by DHHC3 enhances stability and cell surface expression. [UniProt]