ARG62876
anti-CD5 antibody [MEM-32]
anti-CD5 antibody [MEM-32] for ELISA,Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
Developmental Biology antibody; Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [MEM-32] recognizes CD5 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, FACS, IHC-P, IP, WB |
| Specificity | The clone MEM-32 reacts with the cell surface glycoprotein CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T-lymphocytes, most of thymocytes and B-lymphocytes subset (B-1a lymphocytes). HLDA III; WS Code T 523 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-32 |
| Isotype | IgG1 |
| Target Name | CD5 |
| Immunogen | Crude thymus membrane fraction. |
| Conjugation | Un-conjugated |
| Alternate Names | CD antigen CD5; Lymphocyte antigen T1/Leu-1; LEU1; T-cell surface glycoprotein CD5; T1 |
Application Instructions
| Application Suggestion |
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | WB: Under non-reducing condition. ELISA: The clone MEM-32 can be used in the sandwich ELISA as the capture antibody in pair with the detection antibody (clone CRIS1). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||||||||
| Positive Control | IHC-P: Spleen |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD5 |
| Gene Full Name | CD5 molecule |
| Background | CD5 antigen (T1; 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains. The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies. Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (Rheumatoid Arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells. |
| Function | May act as a receptor in regulating T-cell proliferation. [UniProt] |
| Highlight | Related products: CD5 antibodies; CD5 ELISA Kits; CD5 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Lymphoma |
| Research Area | Developmental Biology antibody; Immune System antibody |
| Calculated MW | 55 kDa |
| PTM | Phosphorylated on tyrosine residues by LYN; this creates binding sites for PTPN6/SHP-1. |
Clone References
Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen.
