ARG23060

anti-CD5 antibody [OX-19]

anti-CD5 antibody [OX-19] for IHC-Frozen sections,Flow cytometry,Immunoprecipitation,IHC-Formalin-fixed paraffin-embedded sections and Rat

Overview

Product Description Mouse Monoclonal antibody [OX-19] recognizes CD5
Mouse anti Rat CD5 antibody, clone OX-19 recognizes the rat CD5 cell surface antigen, a 69kD glycoprotein expressed by T cells, thymocytes and a subset of B cells. Mouse anti Rat CD5 antibody, clone OX-19 has been reported as being suitable for use on periodate-lysine paraformaldehyde (PLP) fixed paraffin embedded tissue (Whiteland et al. 1995). Mouse anti Rat CD5 antibody, clone OX-19 is routinely tested in flow cytometry on rat splenocytes.
Tested Reactivity Rat
Tested Application FACS, IHC-Fr, IHC-P, IP
Host Mouse
Clonality Monoclonal
Clone OX-19
Isotype IgG1
Target Name CD5
Antigen Species Rat
Immunogen Rat thymocyte glycoproteins.
Conjugation Un-conjugated
Alternate Names CD antigen CD5; Lymphocyte antigen T1/Leu-1; LEU1; T-cell surface glycoprotein CD5; T1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:25 - 1:100
IHC-FrAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
Application Note IHC-P: PLP fixation is recommended for optimal results.
FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 54236 Rat CD5

Swiss-port # P51882 Rat T-cell surface glycoprotein CD5

Gene Symbol Cd5
Gene Full Name Cd5 molecule
Function May act as a receptor in regulating T-cell proliferation. [UniProt]
Calculated MW 55 kDa
PTM Phosphorylated on tyrosine residues by LYN; this creates binding sites for PTPN6/SHP-1.