ARG42245
anti-CD50 / ICAM3 antibody [MEM-04] (low endotoxin)
anti-CD50 / ICAM3 antibody [MEM-04] (low endotoxin) for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Functional study and Human
Overview
| Product Description | Azide free and low endotoxin Mouse Monoclonal antibody [MEM-04] recognizes CD50 / ICAM3 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, FuncSt, IHC-P |
| Specificity | The antibody MEM-04 reacts with an extracellular epitope of CD50 (ICAM-3), a 120-130 kDa type I membrane protein (immunoglobulin supergene family) expressed on leukocytes, endothelial cells and Langerhans cells; it is negative on platelets and erythrocytes. The MEM-04 blocks DC-SIGN (CD209) binding to ICAM-3 (epitope in D1). |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-04 |
| Isotype | IgG1 |
| Target Name | CD50 / ICAM3 |
| Antigen Species | Human |
| Immunogen | Leukocytes of a patient suffering from LGL-type leukemia. |
| Conjugation | Un-conjugated |
| Alternate Names | CD50; CD antigen CD50; Intercellular adhesion molecule 3; ICAM-3; ICAM-R; CDw50; CDW50 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Purification Note | 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein. |
| Buffer | PBS |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ICAM3 |
| Gene Full Name | intercellular adhesion molecule 3 |
| Background | The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2016] |
| Function | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2) (PubMed:1448173). ICAM3 is also a ligand for integrin alpha-D/beta-2. In association with integrin alpha-L/beta-2, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed:23775590). [UniProt] |
| Cellular Localization | Membrane; Single-pass type I membrane protein. [UniProt] |
| Calculated MW | 60 kDa |
| PTM | Upon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues. N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains. [UniProt] |
