ARG23099
anti-CD51 + CD61 (Integrin alpha V + Integrin beta 3) complex antibody [23C6]
anti-CD51 + CD61 (Integrin alpha V + Integrin beta 3) complex antibody [23C6] for CyTOF®-candidate,Flow cytometry,IHC-Frozen sections,Immunoprecipitation and Chicken,Human
Overview
| Product Description | Mouse Monoclonal antibody [23C6] recognizes CD51 + CD61 (Integrin alpha V + Integrin beta 3) complex. |
|---|---|
| Tested Reactivity | Hu, Chk |
| Species Does Not React With | Bov, Pig, Rb |
| Tested Application | CyTOF®-candidate, FACS, IHC-Fr, IP |
| Specificity | The CD51 + CD61 (Integrin alpha V + Integrin beta 3) complex antibody clone 23C6 recognizes the intact complex formed between the CD51 and CD61 molecules (alpha V and beta 3 integrins). |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 23C6 |
| Isotype | IgG1 |
| Target Name | CD51 + CD61 (Integrin alpha V + Integrin beta 3) |
| Antigen Species | Human |
| Immunogen | Osteoclasts from osteoclastomas. |
| Conjugation | Un-conjugated |
| Alternate Names | CD51; VNRA; CD antigen CD51; VTNR; Vitronectin receptor subunit alpha; Integrin alpha-V; MSK8; GT; CD antigen CD61; CD61; BDPLT2; GPIIIa; BDPLT16; GP3A; Platelet membrane glycoprotein IIIa; Integrin beta-3 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-Fr: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Arigo recommends the use of acetone fixation for frozen sections. FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | ITGAV; ITGB3 |
| Gene Full Name | Integrin alpha V (CD51) Integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) |
| Background | Integrin alpha V (CD51) is a protein that is a member of the integrin superfamily. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein undergoes post-translational cleavage to yield disulfide-linked heavy and light chains that combine with multiple integrin beta chains to form different integrins. This protein has been shown to heterodimerize with beta 1, beta 3, beta 5, beta 6, and beta 8; the heterodimer of alpha v and beta 3 is the Vitronectin receptor. This protein interacts with several extracellular matrix proteins to mediate cell adhesion and may play a role in cell migration. It is proposed that this protein may regulate angiogenesis and cancer progression. Alternative splicing results in multiple transcript variants that encode different protein isoforms. Note that the integrin alpha 5 and integrin alpha V chains are produced by distinct genes. [provided by RefSeq, Jan 2015] The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. [provided by RefSeq, Jul 2008] |
| Function | The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. [UniProt] Integrin alpha-V/beta-3 (ITGAV:ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 (ITGA2B:ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. Fibrinogen binding enhances SELP expression in activated platelets (By similarity). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. [UniProt] |
| Highlight | Related products: CD51 antibodies; Anti-Mouse IgG secondary antibodies; Related news: CyTOF-candidate Antibodies |
| Calculated MW | CD51: 116 kDa CD61: 87 kDa |
| PTM | integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61): Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding. |
