ARG22826

anti-CD52 antibody [YTH34.5] (PE)

anti-CD52 antibody [YTH34.5] (PE) for Flow cytometry and Human,Rhesus Monkey

Overview

Product Description PE-conjugated Rat Monoclonal antibody [YTH34.5] recognizes CD52
Rat anti Human CD52 antibody, clone YTH34.5 reacts with the human CD52 antigen, also known as CAMPATH-1. The CD52 antigen is a remarkably small but heavily glycosylated peptide attached to the cell surface membrane via a GPI link (Xia et al. 1991). The apparent molecular mass of the native antigen on SDS-PAGE is 25-29kDa, considerably reduced following N-glycanase treatment (Rowan et al. 1998).CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.Humanised versions of CAMPATH-1 specific antibodies are currently in clinical trials for the treatment of a range of lymphoid malignancies (Dearden et al. 2002; Pettitt et al. 2012).
Tested Reactivity Hu, R. Mk
Tested Application FACS
Host Rat
Clonality Monoclonal
Clone YTH34.5
Isotype IgG2b
Target Name CD52
Antigen Species Human
Immunogen Human lymphocytes.
Conjugation PE
Alternate Names He5; CAMPATH-1 antigen; Human epididymis-specific protein 5; Cambridge pathology 1 antigen; CDw52; Epididymal secretory protein E5; CDW52; CD antigen CD52

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:20
Application Note FACS: use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.09% Sodium azide, 1% BSA and 5% Sucrose
Preservative 0.09% Sodium azide
Stabilizer 1% BSA and 5% Sucrose
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1043 Human CD52

Swiss-port # P31358 Human CAMPATH-1 antigen

Gene Symbol CD52
Gene Full Name CD52 molecule
Function May play a role in carrying and orienting carbohydrate, as well as having a more specific role. [UniProt]
Calculated MW 7 kDa