ARG62883

anti-CD53 antibody [MEM-53]

anti-CD53 antibody [MEM-53] for Flow cytometry,IHC-Frozen sections,Immunoprecipitation,Western blot,Functional study and Human

Immune System antibody; Signaling Transduction antibody

Overview

Product Description

Mouse Monoclonal antibody [MEM-53] recognizes CD53

Tested Reactivity Hu
Tested Application FACS, FuncSt, IHC-Fr, IP, WB
Specificity The clone MEM-53 reacts with CD53, a 32-40 kDa tetraspanin family glycoprotein exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells.
MEM-53 reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).
HLDA IV; WS Code NL 59
HLDA V; WS Code B CD53.5
HLDA V; WS Code BP BP287
HLDA V; WS Code T T-096
HLDA V; WS Code X XB004
Host Mouse
Clonality Monoclonal
Clone MEM-53
Isotype IgG1
Target Name CD53
Immunogen Leukocytes of pacient suffering from a LGL-type leukemia.
Conjugation Un-conjugated
Alternate Names Tetraspanin-25; Leukocyte surface antigen CD53; Tspan-25; Cell surface glycoprotein CD53; CD antigen CD53; MOX44; TSPAN25

Application Instructions

Application Suggestion
Tested Application Dilution
FACS4 µg/ml
FuncStAssay-dependent
IHC-FrAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note IHC-Fr: It is suitable for discrimination of lymphomas from other tumors.
Functional studies: The clone MEM-53 induces activation of monocytes and B lymphocytes.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 963 Human CD53

Swiss-port # P19397 Human Leukocyte surface antigen CD53

Gene Symbol CD53
Gene Full Name CD53 molecule
Background CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage. This molecule has been reported to form complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4 or tetraspanins CD37, CD81 and CD82, thus probably modulating various signaling processes. CD53 is involved in radioresistancy of tumour cells and its triggering has anti-apoptotic effect. In thymus, CD53 is up-regulated in response to positive selection signals during T cell development, and is strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, whereas stimulation of neutrophils results in down-regulation of CD53 expression.
Function Required for efficient formation of myofibers in regenerating muscle at the level of cell fusion. May be involved in growth regulation in hematopoietic cells (By similarity). [UniProt]
Research Area Immune System antibody; Signaling Transduction antibody
Calculated MW 24 kDa

Clone References

Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells.

Drukker M et al.
Nat Biotechnol.,  (2012)

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CD63 is not required for production of infectious human immunodeficiency virus type 1 in human macrophages.

ICC/IF / 

Ruiz-Mateos E et al.
J Virol.,  (2008)

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In macrophages, HIV-1 assembles into an intracellular plasma membrane domain containing the tetraspanins CD81, CD9, and CD53.

ICC/IF / 

Deneka M et al.
J Cell Biol.,  (2007)

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Cross-linking of CD53 promotes activation of resting human B lymphocytes.

Rasmussen AM et al.
J Immunol.,  (1994)

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