ARG62883
anti-CD53 antibody [MEM-53]
anti-CD53 antibody [MEM-53] for Flow cytometry,IHC-Frozen sections,Immunoprecipitation,Western blot,Functional study and Human
Immune System antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [MEM-53] recognizes CD53 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, FuncSt, IHC-Fr, IP, WB |
| Specificity | The clone MEM-53 reacts with CD53, a 32-40 kDa tetraspanin family glycoprotein exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells. MEM-53 reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F). HLDA IV; WS Code NL 59 HLDA V; WS Code B CD53.5 HLDA V; WS Code BP BP287 HLDA V; WS Code T T-096 HLDA V; WS Code X XB004 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-53 |
| Isotype | IgG1 |
| Target Name | CD53 |
| Immunogen | Leukocytes of pacient suffering from a LGL-type leukemia. |
| Conjugation | Un-conjugated |
| Alternate Names | Tetraspanin-25; Leukocyte surface antigen CD53; Tspan-25; Cell surface glycoprotein CD53; CD antigen CD53; MOX44; TSPAN25 |
Application Instructions
| Application Suggestion |
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | IHC-Fr: It is suitable for discrimination of lymphomas from other tumors. Functional studies: The clone MEM-53 induces activation of monocytes and B lymphocytes. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD53 |
| Gene Full Name | CD53 molecule |
| Background | CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage. This molecule has been reported to form complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4 or tetraspanins CD37, CD81 and CD82, thus probably modulating various signaling processes. CD53 is involved in radioresistancy of tumour cells and its triggering has anti-apoptotic effect. In thymus, CD53 is up-regulated in response to positive selection signals during T cell development, and is strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, whereas stimulation of neutrophils results in down-regulation of CD53 expression. |
| Function | Required for efficient formation of myofibers in regenerating muscle at the level of cell fusion. May be involved in growth regulation in hematopoietic cells (By similarity). [UniProt] |
| Research Area | Immune System antibody; Signaling Transduction antibody |
| Calculated MW | 24 kDa |
Clone References
Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells.
CD63 is not required for production of infectious human immunodeficiency virus type 1 in human macrophages.
ICC/IF /
In macrophages, HIV-1 assembles into an intracellular plasma membrane domain containing the tetraspanins CD81, CD9, and CD53.
ICC/IF /
Cross-linking of CD53 promotes activation of resting human B lymphocytes.
