ARG22605

anti-CD54 / ICAM1 antibody [1A29]

anti-CD54 / ICAM1 antibody [1A29] for IHC-Frozen sections,Flow cytometry,Immunoprecipitation,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Rat

Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody

Overview

Product Description Mouse Monoclonal antibody [1A29] recognizes CD54 / ICAM1
This antibody recognizes the rat CD54 cell surface antigen, also known as intercellular adhesion molecule-1 (ICAM-1), a 90kD adhesion molecule belonging to the immunoglobulin superfamily.CD54 is a cell surface ligand of the lymphocyte integrin, LFA-1 and is known to play an important role in various cell-cell interactions in the immune system. Recent studies suggest that cross-linking of ICAM-1 using clone 1A29 induces calcium signalling (Etienne, S. et al.).Functionally 1A29 inhibits homotypic aggregation of PHA blasts.
Tested Reactivity Rat
Tested Application FACS, IHC-Fr, IHC-P, IP, WB
Host Mouse
Clonality Monoclonal
Clone 1A29
Isotype IgG1
Target Name CD54 / ICAM1
Antigen Species Rat
Immunogen Rat Ax cells (a HEV derived cell line).
Conjugation Un-conjugated
Alternate Names CD54; CD antigen CD54; BB2; P3.58; Intercellular adhesion molecule 1; Major group rhinovirus receptor; ICAM-1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:25 - 1:50
IHC-FrAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note FACS: Use 10ul of the suggested working dilution to label 10^6 cells in 100ul.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 25464 Rat ICAM1

Swiss-port # Q00238 Rat Intercellular adhesion molecule 1

Gene Symbol Icam1
Gene Full Name intercellular adhesion molecule 1
Background This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]
Function ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity). [UniProt]
Research Area Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody
Calculated MW 58 kDa
PTM Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.