ARG56035

anti-CD54 / ICAM1 antibody [1H4]

anti-CD54 / ICAM1 antibody [1H4] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections and Human

Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody

Overview

Product Description Mouse Monoclonal antibody [1H4 ] recognizes CD54 / ICAM1
Tested Reactivity Hu
Tested Application FACS, IHC-P
Host Mouse
Clonality Monoclonal
Clone 1H4
Isotype IgG2b, kappa
Target Name CD54 / ICAM1
Antigen Species Human
Immunogen Raji Burkitt lymphoma cells.
Conjugation Un-conjugated
Alternate Names CD54; CD antigen CD54; BB2; P3.58; Intercellular adhesion molecule 1; Major group rhinovirus receptor; ICAM-1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS0.5 - 1 µg/10^6 cells
IHC-P2 - 4 µg/ml
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0) for 10-20 min, followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3383 Human ICAM1

Swiss-port # P05362 Human Intercellular adhesion molecule 1

Gene Symbol ICAM1
Gene Full Name intercellular adhesion molecule 1
Background This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]
Function ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity). [UniProt]
Cellular Localization Cell surface
Research Area Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody
Calculated MW 58 kDa
PTM Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.