ARG23062
anti-CD54 / ICAM1 antibody [84H10] (FITC)
anti-CD54 / ICAM1 antibody [84H10] (FITC) for Flow cytometry and Human,Dog
Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody
Overview
| Product Description | FITC-conjugated Mouse Monoclonal antibody [84H10] recognizes CD54 / ICAM1 Mouse anti Human CD54 antibody, clone 84H10 recognizes the D1 domain of ICAM-1.It reacts with the ICAM-1 antigen found in low levels on lymphocytes and strongly expressed on monocytes and granulocytes. This molecule is inducible to high levels by mitogenic lectins on lymphocytes and by IL-1 beta or IFN gamma on other cell types such as fibroblasts and endothelial cells. Detects an antigen of 90kD.Mouse anti Human CD54 antibody, clone 84H10 has been reported to block ICAM1 mediated cellular adhesion and block binding of LFA-1 and P. falciparum to ICAM-1.Mouse anti Human CD54 antibody, clone 84H10 is routinely tested in flow cytometry on rat splenocytes. |
|---|---|
| Tested Reactivity | Hu, Dog |
| Tested Application | FACS |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 84H10 |
| Isotype | IgG1 |
| Target Name | CD54 / ICAM1 |
| Antigen Species | Human |
| Immunogen | K562 cell line. |
| Conjugation | FITC |
| Alternate Names | CD54; CD antigen CD54; BB2; P3.58; Intercellular adhesion molecule 1; Major group rhinovirus receptor; ICAM-1 |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by affinity chromatography. |
| Buffer | PBS, 0.1% Sodium azide and 0.2% BSA |
| Preservative | 0.1% Sodium azide |
| Stabilizer | 0.2% BSA |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ICAM1 |
| Gene Full Name | intercellular adhesion molecule 1 |
| Background | This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008] |
| Function | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity). [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody |
| Calculated MW | 58 kDa |
| PTM | Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. |
