ARG62887

anti-CD54 / ICAM1 antibody [MEM-111]

anti-CD54 / ICAM1 antibody [MEM-111] for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Bovine,Human,Rat

Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody

Overview

Product Description

Mouse Monoclonal antibody [MEM-111] recognizes CD54 / ICAM1

Tested Reactivity Hu, Rat, Bov
Tested Application ELISA, FACS, ICC/IF, IHC-Fr, IHC-P, WB
Specificity The clone MEM-111 reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus). The expression of CD54 is upregulated by activation; it is expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.
HLDA VI; WS Code AS A049
Host Mouse
Clonality Monoclonal
Clone MEM-111
Isotype IgG2a
Target Name CD54 / ICAM1
Antigen Species Human
Immunogen Raji human Burkitt's lymphoma cell line
Conjugation Un-conjugated
Alternate Names CD54; CD antigen CD54; BB2; P3.58; Intercellular adhesion molecule 1; Major group rhinovirus receptor; ICAM-1

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACS2 µg/ml
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-P10 ��g/ml
WB1 µg/ml
Application Note WB: Non-reducing condition.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control WB: Raji, K562, JY and activated T-lymphocytes.
IHC-P: Thymus and RE cells

Properties

Form Liquid
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 25464 Rat ICAM1

GeneID: 281839 Bovine ICAM1

GeneID: 3383 Human ICAM1

Gene Symbol ICAM1
Gene Full Name intercellular adhesion molecule 1
Background This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]
Function ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity). [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30035 Phospho CD54 / ICAM1 Antibody Duo (Total, pY512)
Related products:
CD54 antibodies; CD54 ELISA Kits; CD54 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Research Area Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody
Calculated MW 58 kDa
PTM Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.

Clone References

Suramin: a potential therapy for diabetic nephropathy.

Korrapati MC et al.
PLoS One.,  (2013)

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Rhinovirus upregulates transient receptor potential channels in a human neuronal cell line: implications for respiratory virus-induced cough reflex sensitivity.

Abdullah H et al.
Thorax.,  (2014)

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Heterogenic endothelial responses to inflammation: role for differential N-glycosylation and vascular bed of origin.

ELISA / Human

Scott DW et al.
J Am Heart Assoc.,  (2013)

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The use of scaffold-free cell sheet technique to refine mesenchymal stromal cell-based therapy for heart failure.

Narita T et al.
Mol Ther.,  (2013)

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Multipotent Adult Progenitor Cells (MAPC) contribute to hepatocarcinoma neovasculature.

FACS / Horse

Barajas M et al.
Biochem Biophys Res Commun.,  (2007)

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