ARG62891

anti-CD55 antibody [MEM-118]

anti-CD55 antibody [MEM-118] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation and Human,Primates

Immune System antibody

Overview

Product Description

Mouse Monoclonal antibody [MEM-118] recognizes CD55

Tested Reactivity Hu, NHuPrm
Tested Application FACS, IHC-P, IP
Specificity The clone MEM-118 recognizes an epitope in SCR4 domain of CD55 (Decay accelerating factor, DAF), a 60-70 kDa glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein. CD55 is widely expressed on hematopoietic and on many non-hematopoietic cells; it is weakly present on NK cells.
HLDA V; WS Code AS S016
Host Mouse
Clonality Monoclonal
Clone MEM-118
Isotype IgM
Target Name CD55
Antigen Species Human
Immunogen HPB-ALL human T cell line
Conjugation Un-conjugated
Alternate Names DAF; CD antigen CD55; CROM; Complement decay-accelerating factor; CR; TC

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µg/ml
IHC-P10 µg/ml
IPAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from ascites by precipitation methods and size-exclusion chromatography.
Purity > 95% (by SDS-PAGE)
Buffer TBS (pH 8.0) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1604 Human CD55

Swiss-port # P08174 Human Complement decay-accelerating factor

Gene Symbol CD55
Gene Full Name CD55 molecule, decay accelerating factor for complement (Cromer blood group)
Background CD55 (decay-accelerating factor, DAF) is a GPI-anchored membrane glycoprotein that protects autologous cells from classical and alternative pathway of complement cascade. Bidirectional interactions between CD55 and CD97 are involved in T cell regulation and CD55 can still regulate complement when bound to CD97. In tumours, besides protection agains complement, CD55 promotes neoangiogenesis, tumorigenesis, invasiveness and evasion of apoptosis.
Function This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. [UniProt]
Research Area Immune System antibody
Calculated MW 41 kDa
PTM The Ser/Thr-rich domain is heavily O-glycosylated.

Clone References

Exploitation of the complement system by oncogenic Kaposi's sarcoma-associated herpesvirus for cell survival and persistent infection.

ICC/IF / Human

Lee MS et al.
PLoS Pathog.,  (2014)

publication_link

 

hr_line

Disease-specific and inflammation-independent stromal alterations in spondylarthritis synovitis.

IHC / Human

Yeremenko N et al.
Arthritis Rheum.,  (2013)

publication_link

 

hr_line

The nature of large noncovalent complexes containing glycosyl-phosphatidylinositol-anchored membrane glycoproteins and protein tyrosine kinases.

Cinek T et al.
J Immunol.,  (1992)

publication_link

 

hr_line