ARG62890
anti-CD55 antibody [MEM-118] (Biotin)
anti-CD55 antibody [MEM-118] (Biotin) for Flow cytometry and Human,Primates
Immune System antibody
Overview
| Product Description | Biotin-conjugated Mouse Monoclonal antibody [MEM-118] recognizes CD55 |
|---|---|
| Tested Reactivity | Hu, NHuPrm |
| Tested Application | FACS |
| Specificity | The clone MEM-118 recognizes an epitope in SCR4 domain of CD55 (Decay accelerating factor, DAF), a 60-70 kDa glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein. CD55 is widely expressed on hematopoietic and on many non-hematopoietic cells; it is weakly present on NK cells. HLDA V; WS Code AS S016 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-118 |
| Isotype | IgM |
| Target Name | CD55 |
| Antigen Species | Human |
| Immunogen | HPB-ALL human T cell line |
| Conjugation | Biotin |
| Alternate Names | DAF; CD antigen CD55; CROM; Complement decay-accelerating factor; CR; TC |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification Note | The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin. |
| Buffer | TBS (pH 8.0) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P08174 Human Complement decay-accelerating factor |
|---|---|
| Gene Symbol | CD55 |
| Gene Full Name | CD55 molecule, decay accelerating factor for complement (Cromer blood group) |
| Background | CD55 (decay-accelerating factor, DAF) is a GPI-anchored membrane glycoprotein that protects autologous cells from classical and alternative pathway of complement cascade. Bidirectional interactions between CD55 and CD97 are involved in T cell regulation and CD55 can still regulate complement when bound to CD97. In tumours, besides protection agains complement, CD55 promotes neoangiogenesis, tumorigenesis, invasiveness and evasion of apoptosis. |
| Function | This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. [UniProt] |
| Research Area | Immune System antibody |
| Calculated MW | 41 kDa |
| PTM | The Ser/Thr-rich domain is heavily O-glycosylated. |
Clone References
Exploitation of the complement system by oncogenic Kaposi's sarcoma-associated herpesvirus for cell survival and persistent infection.
ICC/IF / Human
Disease-specific and inflammation-independent stromal alterations in spondylarthritis synovitis.
IHC / Human
The nature of large noncovalent complexes containing glycosyl-phosphatidylinositol-anchored membrane glycoproteins and protein tyrosine kinases.
