ARG62894

anti-CD56 / NCAM antibody [MEM-188]

anti-CD56 / NCAM antibody [MEM-188] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation and Human,Primates

Developmental Biology antibody; Neuroscience antibody; General Lymphocyte Marker Study antibody; Natural killer cells antibody

Overview

Product Description Mouse Monoclonal antibody [MEM-188] recognizes CD56 / NCAM
Tested Reactivity Hu, NHuPrm
Tested Application FACS, ICC/IF, IHC-P, IP
Specificity The clone MEM-188 reacts with a 180 kDa isoform of CD56 (NCAM) expressed in leukocytes. It has been suggested that the antibody MEM-188 could react with rhesus monkey lymphocytes. Reactivity with other NCAM isoforms has not been tested.
HLDA VI; WS code A055
HLDA VI; WS Code NK26
HLDA VII; WS code 70077
Host Mouse
Clonality Monoclonal
Clone MEM-188
Isotype IgG2a
Target Name CD56 / NCAM
Antigen Species Human
Immunogen KG-1 human acute myelogenous leukemia cell line
Conjugation Un-conjugated
Alternate Names CD56; NCAM; CD antigen CD56; N-CAM-1; MSK39; NCAM-1; Neural cell adhesion molecule 1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS3 µg/ml
ICC/IF1:50 - 1:100
IHC-P1:50
IPAssay-dependent
Application Note IHC-P: Pretreatment: Heat retrieval (microwave oven) in citrate buffer, standard ABC visualization. Staining technique: Standard ABC technique (DAB+).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control IHC-P: Positive membrane-specific reaction in adjacent peripheral nerve staining of paraprostatic autonomic ganglion.

Properties

Form Liquid
Purification Purified from hybridoma culture supernatant by protein A-affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4684 Human NCAM1

Swiss-port # P13591 Human Neural cell adhesion molecule 1

Gene Symbol NCAM1
Gene Full Name neural cell adhesion molecule 1
Background CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of immunoglobulin family serving as adhesive molecule which is ubiquitously expressed in nervous system, usually as 120 kDa, 140 kDa or 180 kDa isoform, and it is also found on T cells and NK cells. Polysialic modification results in reduction of CD56-mediated cell adhesion and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumours and ovarian granulosa cell tumours.
Function This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. [UniProt]
Highlight Related products:
CD56 antibodies; CD56 ELISA Kits; CD56 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
Lymphoma
Tumor-Infiltrating Lymphocytes (TILs)
Research Area Developmental Biology antibody; Neuroscience antibody; General Lymphocyte Marker Study antibody; Natural killer cells antibody
Calculated MW 95 kDa

Clone References

Fluorescent protein-expressing neural progenitor cells as a tool for transplantation studies.

FACS / Mouse

Skardelly M et al.
PLoS One.,  (2014)

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