ARG53878

anti-CD56 / NCAM antibody [MEM-188] (PE)

anti-CD56 / NCAM antibody [MEM-188] (PE) for Flow cytometry and Human,Primates

Developmental Biology antibody; Neuroscience antibody; General Lymphocyte Marker Study antibody; Natural killer cells antibody

Overview

Product Description PE-conjugated Mouse Monoclonal antibody [MEM-188] recognizes CD56 / NCAM
Tested Reactivity Hu, NHuPrm
Tested Application FACS
Specificity The clone MEM-188 reacts with a 180 kDa isoform of CD56 (NCAM) expressed in leukocytes. It has been suggested that the antibody MEM-188 could react with rhesus monkey lymphocytes. Reactivity with other NCAM isoforms has not been tested.
HLDA VI; WS code A055
HLDA VI; WS Code NK26
HLDA VII; WS code 70077
Host Mouse
Clonality Monoclonal
Clone MEM-188
Isotype IgG2a
Target Name CD56 / NCAM
Antigen Species Human
Immunogen KG-1 human acute myelogenous leukemia cell line
Conjugation PE
Alternate Names CD56; NCAM; CD antigen CD56; N-CAM-1; MSK39; NCAM-1; Neural cell adhesion molecule 1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS20 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4684 Human NCAM1

Swiss-port # P13591 Human Neural cell adhesion molecule 1

Gene Symbol NCAM1
Gene Full Name neural cell adhesion molecule 1
Background CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of immunoglobulin family serving as adhesive molecule which is ubiquitously expressed in nervous system, usually as 120 kDa, 140 kDa or 180 kDa isoform, and it is also found on T cells and NK cells. Polysialic modification results in reduction of CD56-mediated cell adhesion and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumours and ovarian granulosa cell tumours.
Function This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. [UniProt]
Highlight Related products:
CD56 antibodies; CD56 ELISA Kits; CD56 Duos / Panels; Anti-Mouse IgG secondary antibodies;
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Tumor-Infiltrating Lymphocytes (TILs)
Research Area Developmental Biology antibody; Neuroscience antibody; General Lymphocyte Marker Study antibody; Natural killer cells antibody
Calculated MW 95 kDa

Clone References

Fluorescent protein-expressing neural progenitor cells as a tool for transplantation studies.

FACS / Mouse

Skardelly M et al.
PLoS One.,  (2014)

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