ARG10846

anti-CD57 antibody [E20-I]

anti-CD57 antibody [E20-I] for IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat

Overview

Product Description

Rabbit Monoclonal antibody [E20-I] recognizes CD57

Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P
Host Rabbit
Clonality Monoclonal
Clone E20-I
Target Name CD57
Antigen Species Human
Immunogen Synthetic peptide around the C-terminus of Human CD57.
Conjugation Un-conjugated
Alternate Names Glucuronosyltransferase P; CD57; LEU7; GlcAT-P; GLCATP; HNK1; GlcUAT-P; NK1; Beta-1,3-glucuronyltransferase 1; EC 2.4.1.135; Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1; GLCUATP; UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase; NK-1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:200
Application Note IHC-P: Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.0) for 25-35 min followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer 20 mM Tris-HCl (pH 8.0), 0.05% Sodium azide and 20 mg/ml BSA.
Preservative 0.05% Sodium azide
Stabilizer 20 mg/ml BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 117108 Rat B3GAT1

GeneID: 27087 Human B3GAT1

Swiss-port # O35789 Rat Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1

Swiss-port # Q9P2W7 Human Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1

Gene Symbol B3GAT1
Gene Full Name beta-1,3-glucuronyltransferase 1
Background The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]
Function Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group (By similarity). [UniProt]
Calculated MW 38 kDa
PTM The soluble form derives from the membrane form by proteolytic processing. [UniProt]